微小隐孢子虫SA35与SA40蛋白的B细胞抗原表位预测  

Prediction of B-Cell Epitopes on SA35 and SA40 of Cryptosporidium parvum

作　　者：单昊[1] 徐进梅[1] 侯敏[1] 季旻珺[1] 张素华[1] 吴海玮[1] 

机构地区：[1]南京医科大学病原生物学系,江苏省病原生物学重点实验室,南京210029

出　　处：《热带医学杂志》2007年第6期509-512,528,共5页Journal of Tropical Medicine

基　　金：江苏省病原生物学重点实验室开放课题(No.Y2005)

摘　　要：目的预测微小隐孢子虫SA35和SA40蛋白的B细胞表位。方法以单参数(亲水性、可及性、柔韧性及抗原性)预测为基础,通过二级结构预测初步筛选,并以ABCpred方案作为最终验证,预测微小隐孢子虫SA35和SA40蛋白的B细胞表位。结果SA35蛋白N端103～115和129～146和以及SA40蛋白的N端77～89、127～136、156～174和200～209区段为预测的B细胞表位。结论所得表位为这两种蛋白以后应用于合成肽检测、制备相应的抗体、发展高特异性和敏感性的诊断系统以及研制疫苗等提供了理论基础。Objective To predict B-cell epitopes on SA35 and SA40 of Cryptosporidium parvum. Method The location of B-cell epitopes was predicted by screening the secondary structure of SA35 and SA40. Analysis was on the basis of the prediction results of Parker Hydrophilicity, Emini Accessibility, Karplus ＆ Schulz Flexibility, and Kolaskar ＆ Tongaonkar Antigenicity followed by a verification using ABCpred program. Result The predicted B-cell epitopes were found to locate at the protein sequence of amino acids 273-278, 103-115, 129-146 and 31-37 for SA35, and 77-89, 127-136, 156-174 and 200-209 for SA40. Conclusion The information may be used in the peptide synthesis, preparation of anti-SA35 and anti-SA40 antibodies, and the development of specific diagnositic reagents for Cryptosporidium parvum.

关 键 词：微小隐孢子虫 SA35蛋白 SA40蛋白 B细胞表位 预测 ABCpred 

分 类 号：R382.3[医药卫生—医学寄生虫学]