转乙肝病毒表面抗原基因烟草发根的获得  

Transformation of HBsAg Gene into Tobacco and Production of Transgenic Tobacco Hairy Roots

作　　者：王逸群[1] 李田[1] 李文明[1] 

机构地区：[1]福建师范大学生命科学学院发育与神经生物学福建省高校重点实验室,福建福州350108

出　　处：《吉首大学学报（自然科学版）》2007年第3期95-98,共4页Journal of Jishou University(Natural Sciences Edition)

基　　金：福建省自然科学基金资助项目(B0410009);福建省科技厅资助项目(2004N026);福建省教育厅科学研究项目(JB03125)

摘　　要：构建了乙肝病毒表面抗原基因(HBsAg)植物表达载体,通过冻融法将HBsAg基因转入到发根农杆菌LBA1314中,采用叶盘法将HBsAg基因导入到烟草中,获得了转基因烟草发根.对转基因烟草发根的GUS检测结果表明:转HBsAg基因烟草发根可以染成蓝色,而非转基因烟草的根没有染色反应.这说明gus基因在转HBsAg基因烟草发根获得了表达.A plant expression carrier containing HBsAg （hepatitis B virus surface antigen） gene was constructed in a plasmid, the plasmid was mobilized into a rhizogenes Strain LBA 1314 by freeze-thaw method, the HBsAg gene was planted into tobacco by leaf disc via Agrobacterium tumefaciens LBA 4404 as a vehicle and the tobacco hairy roots were received. Histochemical staining of GUS activity was made in the transgenic tobacco hairy roots： The roots with HBsAg genes can be dyed into bule, while those without have no reflection of color. So the conclusion can acquire that the gene has its expression in the roots.

关 键 词：乙肝病毒表面抗原基因 烟草 发根 转化 

分 类 号：A785[哲学宗教—马克思主义哲学]