胚龄、NAA浓度、基因型对杂交小麦及其亲本幼胚培养的影响  

Effects of Embryo Age,NAA and Genotype Difference on the Tissue Culture of Immature Embryos from Hybrid Wheat and Its Parents

作　　者：栗现芳[1] 马守才[1] 张改生[1] 牛娜[1] 

机构地区：[1]西北农林科技大学陕西省作物杂种优势研究与利用重点实验室,陕西杨凌712100

出　　处：《河南农业科学》2007年第6期31-34,共4页Journal of Henan Agricultural Sciences

基　　金：陕西省"十五"科技攻关项目(2004-2005K01-G4);国家杨陵农业生物技术育种中心专项基金项目(99-1A)

摘　　要：以杂交小麦西杂一号、西杂五号及其亲本Fp1,Mp1,Fp2,Mp2为试材,采用幼胚培养一步成苗法研究了胚龄、NAA浓度、基因型对小麦幼胚培养成苗率及生长情况的影响。结果表明,幼胚培养最适宜的胚龄为16 d;最适宜的NAA浓度为0.13 mg/L;杂交小麦幼胚出苗率明显高于自交小麦幼胚,自交小麦品种中Fp2培养成苗效果较佳。因此,胚龄、NAA浓度及基因型对小麦幼胚组织培养具有明显的调节作用。在实践中应协调这些因素的作用,提高组织培养效率,从而缩短育种年限,加速育种进程。As an experimental material, XZ1, XZ5 and their parents, uses immature embryos of wheat tissue culture. The effects of age of immature embryos, NAA and different wheat cultivars on the frequency of seedling and growth of the tissue culture from immature embryos of wheat were reported in this paper. The experiment results indicated that the age of immature embryos for its tissue culture was 16d; The optimum concentration of NAA was 0. 130mg/L; The rate of germination of hybrid wheat embryo significantly higher than that of self-wheat embryo; In self-wheat varieties,Fp2 results better cultured seedlings. From all these accounts, we can safely say that the age of immature embryos, concentration of NAA and genotype obviously took regulative effects on the tissue culture from immature embryos of wheat. In practice, the integrating of these effects was not only helpful to increase the efficiency of the tissue culture from immature embryos of wheat, but also helpful to shorten the breeding period and promote the breeding process.

关 键 词：小麦 胚龄 NAA 幼胚 组织培养 

分 类 号：S512.2[农业科学—作物学]