人尿中酚、粘糠酸、苯巯基尿酸的生物监测  

Biological monitoring of low levels of benzene exopsure

作　　者：吴宜群[1,2,3] 唐瑾[1,2,3] 程玉琪 李春玲[1,2,3] 李晓华[1,2,3] 曲宁[1,2,3] 虞爱如 祖静华[1,2,3] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所 [2]辽宁省劳动卫生研究所 [3]北京焦化厂

出　　处：《中华劳动卫生职业病杂志》1997年第3期134-137,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：为了探索接触低浓度苯的生物监测指标，在建立了灵敏、特异的尿中反，反－粘糠酸（ｔ，ｔ－ＭＡ）的高效液相色谱（ＨＰＬＣ）、苯巯基尿酸（Ｓ－ＰＭＡ）的色谱／质谱／质谱（ＬＣ／ＭＳ／ＭＳ）测定方法的基础上，对４９位接触低浓度苯的工人及２０位非职业接触者进行了生物监测。结果表明：在苯接触浓度低于３．２ｍｇ／ｍ３（１ｐｐｍ）的情况下，作业工人的尿ｔ，ｔ－ＭＡ和Ｓ－ＰＭＡ浓度与空气中苯的ＴＷＡ浓度显著相关；尿酚与空气中苯的ＴＷＡ浓度之间的相关性很差。吸烟能增加尿ｔ，ｔ－ＭＡ和Ｓ－ＰＭＡ的浓度。The methods for determining urinary t,t muconic acid(t,t MA) by HPLC and S phenylmercapturic acid(S PMA) by LC/MS/MS were developed and successfully applied to urine samples from 49 workers exposed to benzene and 20 non exposed controls.The t,t MA and S PMA concentrations in urine correlate linearly with time weighted average benzene concentration in the breath zone air of workers.When benzene concentration in air was below 3.2 mg/m 3(1 ppm),urinary t,t MA and S PMA were far more specific than phenol.Urinary t,t MA and S PMA can be proposed as suitable indicators for the monitoring of benzene exposure concentrations down to 3.2 mg/m 3 level.

关 键 词：粘糠酸 苯巯基尿酸 酚 苯 生物监测 生物标志物 

分 类 号：R598.120.1[医药卫生—内科学] R446.122[医药卫生—临床医学]