三氯乙烯诱发小鼠及人外周血有核细胞 DNA 链断裂  

Induction of DNA strand breaks by trichloroethylene in mouse and human lymphocytes in vivo

作　　者：唐国慧[1,2,3] 庄志雄[1,2,3] 张锦周 

机构地区：[1]西安医科大学预防医学系 [2]中山医科大学公共卫生学院 [3]深圳市卫生防疫站

出　　处：《中华劳动卫生职业病杂志》1997年第3期146-149,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：美国中华医学基金

摘　　要：为研究三氯乙烯（ＴＣＥ）的遗传毒性，应用单细胞凝胶电泳法（ＳＣＧＥ）测定了ＴＣＥ诱导人和小鼠外周血有核细胞ＤＮＡ链断裂的能力。小鼠ＴＣＥ染毒剂量为５．８ｍｍｏｌ／ｋｇ时，可见其断裂的ＤＮＡ片断在电场中迁移形成的“彗星”影像长度显著增加，并表现出明显的剂量－效应关系；ＴＣＥ职业暴露人群的外周血有核细胞ＤＮＡ有轻微损伤，但与对照组的差异无显著性，可能与调查对象接触ＴＣＥ时间较短有关。ＳＣＧＥ有可能成为评价化学毒物诱导ＤＮＡ损伤的敏感的、低损害的检测技术。The ability of trichloroethylene(TCE) to induce single strand breaks in peripheral lymphocytes DNA of male mice in vivo was evaluated using single cell gel electrophoresis(SCGE).The results showed that TCE was able to induce DNA damage in a dose dependent manner in the range of 5.8￣ 22.9 mmol/kg.TCE had a positive genotoxic effect.However,no single strand breaks were observed in the study on the workers exposed to TCE for a short period.SCGE has high sensitivity in measuring DNA damage in individual cell and thus possibly become a valuable tool in predicting the genotoxicity of compounds of interest.

关 键 词：三氯乙烯 单细胞凝胶电泳 DNA损伤 生物标志物 

分 类 号：R598.04[医药卫生—内科学] R991[医药卫生—临床医学]