氡及其子体诱发大鼠体细胞HPRT基因位点的突变  

Measuring the HPRT Gene Mutation in Rat after Exposure to Radon and Its Daughters

作　　者：崔凤梅[1] 宁萍[1] 段蓉芳[1] 童建[1] 

机构地区：[1]苏州大学 放射医学与公共卫生学院江苏省放射医学与防护重点实验室,江苏苏州215123

出　　处：《环境与职业医学》2007年第3期282-284,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金(编号:30371226);江苏省高校基础研究项目(编号:05KJA33013);苏州大学青年教师基金(编号:Q3126534)

摘　　要：[目的]用多核细胞法研究氡及其子体诱发大鼠外周血淋巴细胞和气管-支气管上皮细胞的次黄嘌呤鸟嘌呤磷酸核糖转移酶(HPRT)基因位点突变情况。[方法]Wistar雄性大鼠24只,采用HD-3型多功能移动式氡室进行动态吸入染毒,按照氡染毒的累计暴露量,分为3个实验组和1个对照组;获取大鼠外周血淋巴细胞和气管-支气管上皮细胞,以多核细胞法检测该两种细胞的HPRT基因突变频率。[结果]大鼠外周血淋巴细胞和气管-支气管上皮细胞的HPRT基因突变频率均随氡累积暴露剂量的增加而相应增加,剂量-效应关系明显,拟合的函数分别为(?)=1.785×10^(-5)D^2+ 1.174×10^(-3)D+1.054和(?)=3.538×10^(-5)D^2+8.338×10^(-4)D+1.032。[结论]氡及其子体能诱发大鼠外周血淋巴细胞和气管-支气管上皮细胞的HPRT基因突变,呈现一定的剂量-效应关系。[ Objective ] The HPRT gene mutation was studied in rat after inhaling radon and its daughters using the muhinucleated cells method. [ Methods ] 24 Wistar male rats were divided into 4 groups evenly by random. When the accumulated dose Of Radon and its daughters reached to the expected dose, the rats were anesthetized to get the peripheral blood lymphocytes and trachea-bronchia epithelial cells. The HPRT gene mutation test was performed using the muhinucleated cells method. [ Results ] The results of the HPRT gene test indicated that the mutation frequencies （ MFs ） of HPRT gene of the peripheral blood lymphocytes and trachea-bronchia epithelial tells of Radon inhaled groups were all higher than the control respectively （P〈 0.05 ）. Correlation assay showed there have no correlation between the traehea-bronchia epithelial cells and peripheral blood lymphocytes on MFs-HPRT（ P 〉 0.05 ）. [ Conclusion ] Radon could cause increasing of MFs-HPRT in rat peripheral blood lymphocytes and trachea-bronchial epithelial cells.

关 键 词：氡及其子体 外周血淋巴细胞 气管-支气管上皮细胞 HPRT基因位点突变 

分 类 号：R114[医药卫生—卫生毒理学]