内源性一氧化碳参与呼吸节律调节  

Involvement of endogenous carbon monoxide in regulation of respiratory rhythm in vitro

作　　者：杨文星[1] 张奇兰[1] 呼海燕[1] 刘谨[1] 李永波[1] 周华[1] 郑煜[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,成都610041

出　　处：《生理学报》2007年第3期325-330,共6页Acta Physiologica Sinica

基　　金：the National Natural Science Foundation of China(No.30370530);the Specialized ResearchFund for the Doctoral Program of Higher Education of China(No.20060610092)

摘　　要：本文旨在探讨内源性一氧化碳(carbonmonoxide,CO)对呼吸节律的调节作用。采用新生Sprague-Dawley大鼠,制备离体延髓脑片标本,分别灌流CO、血红素氧合酶(hemeoxygenase,HO)底物高铁血红素(hemin)和HO抑制剂ZnPP-9,观察舌下神经根呼吸样传出放电节律的变化。实验分为5组:单纯人工脑脊液(artificial cerebrospinal fluid,ACSF)对照组、ZnPP-9组、外源性CO组、Hemin组和ZnPP-9+Hemin组。结果如下:在ZnPP-9组,舌下神经根节律性放电频率(discharge frequency,DF)增快(P<0.05);在外源性CO组,舌下神经根节律性DF减慢(P<0.05);在Hemin组和ZnPP-9+Hemin组,舌下神经根节律性DF增快(P<0.05)。结果表明,内源性CO对呼吸节律可能具有调节作用。The aim of the present study was to investigate the effect of endogenous carbon monoxide （CO） on respiratory rhythm. The experiments were carried out on the medullary slices of newborn Sprague-Dawley rats. The rhythmic discharge frequency （DF） of hypoglossal rootlets was taken as an index of rhythmic respiratory activity. The slices of medulla oblongata were superfused with ZnPP-9 （inhibitor of heine oxygenase）, CO and hemin （substrate of heine oxygenase）, respectively, to observe their effects on respiratory rhythm. The preparations were divided into 5 groups： control group of artificial cerebrospinal fluid （ACSF）, group of ZnPP-9, group of exogenous CO, group of hemin and group of ZnPP-9 ＋ hemin. The results obtained were as follows. In ZnPP-9 group, the rhythmic DF of the hypoglossal rootlets was increased （P〈0.05）; while in exogenous CO group, it was decreased （P〈0.05）. In the groups of hemin and ZnPP-9 ＋ hemin, the rhythmic DF of the hypoglossal rootlets was increased （P〈0.05）. It is suggested that endogenous CO may play an important role in the regulation of respiratory rhythm.

关 键 词：一氧化碳 血红素氧合酶 呼吸 离体 新生大鼠 

分 类 号：R332.3[医药卫生—人体生理学] Q473[医药卫生—基础医学]