Cdc42和球形肌动蛋白在卵母细胞胞质分裂中的定位分析  

Colocalization of Cdc42 and G-actin during Xenopus Oocyte Cytokinesis

作　　者：程大也[1] 梁彬[1] 李丰[1] 

机构地区：[1]中国医科大学细胞生物学卫生部重点实验室

出　　处：《细胞生物学杂志》2007年第3期415-419,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金资助项目(No.30370736;No.30570966);教育部博士点基金(No.20050159023);教育部回国人员科研启动基金资助

摘　　要：研究活性Cdc42与球形肌动蛋白(G-actin)在爪蟾卵母细胞胞质分裂中的定位关系。分别用GFP-wGBDmRNA与罗丹明-594-微管蛋白、Alexa-488-球形肌动蛋白与罗丹明-594-微管蛋白、GFP-wGBDmRNA与Alexa-594-球形肌动蛋白共同显微注射爪蟾卵母细胞。利用共聚焦显微镜,时间延迟摄影方法,分别观察活体卵母细胞中活性Cdc42、球形肌动蛋白在胞质分裂过程中的定位,以及活性Cdc42与球形肌动蛋白在胞质分裂中的定位关系。在卵母细胞胞质分裂中,活性Cdc42与球形肌动蛋白存在空间上共定位现象,并且在时相上具有一致性。结果提示活性Cdc42和球形肌动蛋白在卵母细胞胞质分裂过程中密切相关。Study the colocalization of active Cdc42 and G-actin during Xenopus oocyte cytokinesis. GFP- wGBD mRNA and rhodamine-594-tubulin, alexa-488-G-actin and rhodamine-594-tubulin, GFP- wGBD mRNA and alexa-594-G-actin were microinjected into Xenopus oocytes respectively. Employ confocal microscopy and timelapse experiments to observe localization of active Cdc42 and G-actin as well as colocalization of active Cdc42 and G-actin during oocyte cytokinesis. Results showed that active Cdc42 colocalized with G-actin spatially and temporally, and indicated active Cdc42 might tightly correlated with G-actin during Xenopus oocyte division processs.

关 键 词：CDC42 球形肌动蛋白 卵母细胞 胞质分裂 

分 类 号：Q132.1[生物学—普通生物学]