游离脂肪酸对脂肪细胞分化的影响  

Effects and mechanisms of free fatty acid on adipocyte differentiation

作　　者：俞静[1] 刘娟[1] 孙岩[1] 刘云[1] 丁国宪[1] 

机构地区：[1]南京医科大学第一附属医院老年医学科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第7期661-665,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省135医学重点人才基金资助(30570878)

摘　　要：目的:探讨游离脂肪酸(free fatty acid,FFA)对3T3-L1前脂肪细胞分化的影响及其可能的作用机制。方法:用FFA(100μg/ml)刺激小鼠成纤维细胞株3T3-L1;构建11β-羟化类固醇脱氢酶1(11β-hydroxysteroid dehydrogenase type1,11β-HSD1)的SiRNA表达质粒pGCsilencerTM H1/TetO1-11β-HSD1转染到3T3-L1细胞中并稳定表达,Western blot验证转染效率;采用荧光定量PCR检测脂肪细胞分化相关标志基因:过氧化物酶体增殖物激活受体γ(Peroxisome proliferator-activated receptorγ,PPARγ),CAATT增强子结合蛋白α(CAATT enhancer binding proteins α,C/EBPα),脂蛋白脂酶(Lipoprotein lipase,LPL)和脂肪酸合成酶(Fatty acid synthetase,FAS)mRNA表达量的改变以及FFA刺激时11β-HSD1的基因表达水平。结果:FFA刺激后,分化指标PPARγ、C/EBPα、LPL和FAS的mRNA表达量增加,促进了脂肪细胞的分化(P<0.01);同时11β-HSD1基因的表达增加(P<0.05);11β-HSD1基因沉默后,分化指标的mRNA表达量降低,脂肪细胞的分化能力下降(P<0.01)。结论:FFA和11!-HSD1都能促进脂肪细胞分化,FFA的成脂作用可能通过增加11!-HSD1的表达实现。Objective：To investigate the effects and mechanisms of free fatty acid （FFA） on 3T3-L1 preadipocyte differentiation. Methods： 1 β-HSD1-SiRNA expression plasmid （pGCsilencerTM H1/TetOl-1β-HSD1） was constructed and transfected into 3T3-L1 cells. 100 μg/ml FFA was added to the 3T3-L1 cells. Levels of adipogenesis-associated markers such as Peroxisome proliferatoractivated receptorγ （PPARγ）, CCAAT enhancer binding protein α （C/EBPα）, lipoprotein lipase （LPL）, and fatty acid synthetase （FAS） were measured. Results：When the cells treated with FFA, mRNA expressions of PPARγC/EBPα,LPL and FAS were upregulated and the expression of 11β-HSD1 also increased. When 11β-HSD1 was silenced, the levels of the markers were downregulated. Condusion：FFA and 11β-HSD1 could promote adipocyte differentiation, and the effect of FFA on adipogenesis carried out through increasing the expression of 1 β-HSD1.

关 键 词：11β-羟化类固醇脱氢酶1 游离脂肪酸 3T3-L1细胞 脂肪细胞分化 

分 类 号：R392.12[医药卫生—免疫学]