低氧对大鼠睾丸生殖细胞凋亡的影响  

Hypoxia Promotes Apoptosis of Germ Cells in Rat Testes

作　　者：廖卫公[1] 高钰琪[1] 蔡明春[1] 吴艺[1] 黄瑊[1] 范有明[1] 

机构地区：[1]第三军医大学高原军事医学系病理生理学与高原生理学教研室全军高原医学重点实验室,重庆400038

出　　处：《中华男科学杂志》2007年第6期487-491,共5页National Journal of Andrology

基　　金：国家自然科学基金(30393131)

摘　　要：目的:研究低氧对大鼠睾丸生殖细胞凋亡和Bax、Bcl-2表达的影响。方法:雄性成年Wistar大鼠随机分为4组:常氧对照组、低氧5 d组、低氧15 d组和低氧30 d组(各组n=6)。常氧对照组在平原喂养;低氧5 d组、15 d组和30 d组分别在低压舱内模拟5 000 m高原喂养5、15、30 d。采用流式细胞术和TUNEL法检测低氧对睾丸生殖细胞凋亡的影响。运用Western印迹技术检测低氧对大鼠睾丸内凋亡相关蛋白Bax、Bcl-2表达的影响。结果:低氧5 d组、15 d组和30 d组睾丸内发生生殖细胞凋亡的生精小管数量[每100个生精小管中,含有凋亡生殖细胞的生精小管数分别为(20.50±5.07)、(21.25±7.85)、(14.00±2.45)个]均非常显著地高于常氧对照组[(6.00±2.16)个,P<0.01]。凋亡的生殖细胞以精原和精母细胞为主。低氧15 d组睾丸组织亚单倍体细胞百分率[(2.18±0.82)%]显著高于常氧对照组[(1.30±0.33)%,P<0.05],低氧30 d组[(3.08±0.93)%]极显著高于常氧对照组(P<0.01)。低氧30 d组睾丸组织Bax表达显著高于常氧对照组(P<0.05),其灰度值分别为17.34±4.54和10.50±2.82。低氧30 d组睾丸组织Bax/Bcl-2比值显著高于常氧对照组(P<0.01),比值分别为0.40±0.10和0.27±0.04。结论:低氧诱导大鼠睾丸生殖细胞凋亡增多,慢性低氧引起的睾丸生殖细胞凋亡增多与Bax表达增加有关。Objective ： To explore the effects of hypobaric hypoxia on the apoptosis of germ cells in male rats. Methods ： Adult male Wistar rats were randomly divided into four groups： a control group raised at sea level; a 5 d, a 15 d and a 30 d hypoxic group raised in a hypobaric chamber simulating 5 000 m altitude for 5 days, 15 days and 30 days respectively. Flow cytometry and TUNEL were used to evaluate the apoptosis of germ cells in the testis. Bax and Bcl-2 in the testis were measured by Western blot. Results ： Seminiferous tubules with apoptotic germ cells were significantly more in the hypoxic groups than in the control （ P 〈 0.01 ）. Most apoptotic germ cells were spermatogonia and spermatocytes. Compared with the control group, apoptotic germ cells detected by PI flow cytometry were significantly increased in the hypoxic 15 d and 30 d groups（ P 〈 0.05 ） ; Bax was significantly higher（ P 〈 0.05 ）, and so was the ratio of Bax to Bcl-2 in the hypoxic 30 d group （ P 〈 0.01 ）. Conclusion ： Hypoxia promotes apoptosis of testicular germ cells in male rats. Chronic hypoxia increases Bax expression in the rat testis.

关 键 词：细胞凋亡 低氧 生殖细胞 BAX BCL-2 大鼠 

分 类 号：Q492[生物学—生理学]