卡介苗对哮喘小鼠气道炎症、T_H细胞分化及血清OVA特异性IgE的影响  

Effects of Bacillus Calmette-Guerin on airway inflammation, T helper cell differentiation and serum OVA-specific IgE levels in a mouse model of asthma

作　　者：陈静璐[1] 沈华浩[1] 赵伟和[2] 

机构地区：[1]浙江大学医学院附属第二医院呼吸科 [2]宁波市第二医院呼吸科

出　　处：《中华微生物学和免疫学杂志》2007年第6期574-578,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨卡介苗(BCG)对哮喘小鼠气道炎症、T_H细胞分化及血清OVA特异性IgE的影响。方法动物随机分为阴性对照组、OVA致敏激发组和BCG干预组。检测气管血管旁嗜酸性粒细胞(EOS)数目、气管上皮杯状细胞增生指数和黏液储存指数、小鼠外周血上清液中细胞因子白细胞介素5(IL-5)及γ干扰素(IFN-γ)浓度、小鼠肺组织中T-bet和GATA-3 mRNA水平及小鼠血清中OVA特异性IgE表达。结果BCG干预组和OVA致敏激发组中小鼠肺气管血管旁单位面积EOS数目分别为(65.09±32.58)个/mm^2、(941.86±592.03)个/mm^2,两者比较差异有统计学意义(P<0.05);BCG干预组和OVA致敏激发组杯状细胞增生指数分别为(19.25±12.27)个/mm、(54.39±24.50)个/mm,BCG干预组和OVA致敏激发组黏液储存指数分别为(6.47±3.94)%、(15.53±11.38)%,两者比较差异有统计学意义(P<0.001);BCG干预组和OVA致敏激发组IL-5浓度分别为(83.40±11.33)pg/ml、(192.30±44.30)pg/ml,IFN-γ浓度分别为(28.69±6.05)pg/ml、(18.12±3.78)pg/ml,T-bet/GATA-3 mR- NA比值分别为1.02±0.18、0.74±0.07,两者比较均差异有统计学意义(P<0.01);BCG干预组和OVA致敏激发组血清中OVA特异性IgE的表达分别为0.61±0.27、1.05±0.45,两者比较差异有统计学意义(P<0.05)。结论BCG能抑制哮喘小鼠气道EOS浸润和黏液高分泌状态,影响T-bet/GATA-3表达水平,降低IL-5水平而增高IFN-γ水平,并能抑制哮喘小鼠血清中OVA特异性IgE的表达。Objective To investigate the effects of Bacillus Calmette-Guerin（BCG） on airway inflammatian , T helper cell differenfiation and serum OVA - specific IgE levels in a mouse model of asthma. Methods C57BL/6 mice were sensitized and challenged by ovabumin （OVA） as positive group（ n = 8）, and mice were treated by saline as a negative group（ n = 6）. Mice were infected with BCG two weeks before the first OVA challenge as BCG treated group（ n = 8）. Peribronchial and perivascular eosinophilia, goblet cell hyperplasia index and mucus overproduction index, IL-5 and 1FN-7 concentrations in the supernatant, T-bet and GATA-3 mRNA expression levels in lung tissue and senun OVA-specific IgE levels were tested. Results Peribronchial and perivascular easinophilia of BCG treated group was （65.09 ± 32.58）/mm^2, which was significantly depressed compared to that in positive group （941.86 ± 592.03）/mm^2 （ P 〈 0.05）. Goblet cell hyperplasia index in BCG treated group was lower than that in positive group [（19.25±12.27）/mm vs （54.39 ± 24.50）/mm, P 〈 0.001 ] ; mucus overproduction index in BCG treated group was also lower than that in positive group [ （6.47 ± 3.94）% vs （ 15.53 ± 11.38）%, P 〈0.001]. The concentration of IL-5 was lower in BCG treated group than that in positive group [（83.40±11.33） pg/ml vs （192.30±44.30） pg/ml, P〈0.01]. The concentration of IFN-γ was higher in BCG treated group than that in positive group [ （28.69 ± 6.05） pg/ml vs （ 18.12 ± 3.78） pg/ml, P 〈 0.01 ]. The ratio of T- bet and GATA-3 mRNA was higher in BCG treated group than that in positive group [（1.02± 0.18） vs （0.74± 0.07）, P 〈0.01]. The level of serum OVA-specific IgE was lower in BCG treated group as comparing group [（0.61 ± 0.27） vs （1.05 ± 0.45）, P 〈 0.05]. Conclusion These findings suggest that BCG can inhibit airway eosinophilia and mucus overproduction, influence the expression of T-bet and GATA-3, depress the expression of IL-5, elevate the

关 键 词：哮喘 卡介苗 气道炎症 TH细胞分化 血清OVA特异性IgE 

分 类 号：R562.25[医药卫生—呼吸系统]