肿瘤坏死因子α和罗格列酮对3T3-L1细胞分化过程中蛋白酪氨酸磷酸酶1B表达的影响  

Effects of tumor necrosis factor-αand rosiglitazone on the expression of protein tyrosine phosphatase 1B during the differentiation of 3T3-L1 preadipocytes

作　　者：陈月[1] 邹大进[1] 张征[2] 王淼[1] 吴捷[1] 

机构地区：[1]第二军医大学长海医院内分泌科,上海200433 [2]解放军四一一医院内分泌科

出　　处：《中华内分泌代谢杂志》2007年第3期275-278,共4页Chinese Journal of Endocrinology and Metabolism

基　　金："十五"攻关基金(2001BA70801)

摘　　要：目的观察3T3-L1前脂肪细胞分化过程中蛋白酪氨酸磷酸酶1B(PTP1 B)蛋白水平的变化以及肿瘤坏死因子α(TNF-α)和罗格列酮在前脂肪细胞分化过程中对PTP1B表达的影响,探讨PTP1B在脂肪细胞分化中的作用。方法体外培养3T3-L1前脂肪细胞,分别采用3组诱导剂(完全诱导剂:3-异丁基-1-甲基黄嘌呤+地塞米松+胰岛素,C组),完全诱导剂加20μg/L TNF-α(CT组),完全诱导剂加10^(-3) mol/L罗格列酮(CR组)诱导脂肪细胞分化,以Western印迹方法检测各组脂肪细胞分化过程中PTP1B蛋白表达变化。结果3组中PTP1B蛋白均表现为在前脂肪细胞中(第1天)表达最高,随着脂肪细胞分化成熟表达逐步降低,至第10天降至最低;与C组相比,CT组中脂肪细胞分化较为迟缓,其分化后期(第7～10天)PTP1B蛋白表达水平较C组显著增高;而CR组则表现为脂肪细胞分化活跃,其分化后期(第7～10天)PTP1B蛋白表达水平较C组显著降低。结论在脂肪细胞分化成熟过程中PTP1B蛋白表达呈降低趋势;TNF-α及罗格列酮影响脂肪细胞胰岛素敏感性的作用可能与其调控PTP1B的表达有关。Objective To investigate the protein level of protein tyrosine phosphatase 1 B （ PTP1 B） during the differentiation of 3T3-L1 preadipocytes and the effects of tumor necrosis factor-α （TNF-α） and rosiglitazone on the expression of PTP1B during the differentiation, and to explore the relationship between PTP1 B and adipoeyte differentiation. Methods 3T3-L1 preadipocytes were cultured in vitro and differentiated by three groups of inducers ： basic inducers only （3-isobutyl-1-methylxanthine ＋ dexamethasone ＋ insulin, group C ） , 20 μg/L TNF-α ＋ basic inducers （group CT） and 10^-5 mol/L rosiglitazone ＋ basic inducers （group CR）. Protein level of PTP-1B in adipocytes during differentiation was detected by Western blot. Results Each group showed the relatively high level of PTP1B in 3T3-L1 preadipocytes, going down with the differentiation of adipocytes, and reaching the lowest level in fully-matured adipocytes. Comparing the late period of differentiation in these three groups, CT group was sluggishly differentiated with more PTP1B protein, and CR group showed active differentiation with the lowest level of PTP1B. Conclusion PTP1B decreases with the differentiation of adipocytes. The effects of TNF-α and rosiglitazone on insulin sensitivity perhaps partly via their influences on PTP1 B level in adipocytes.

关 键 词：蛋白酪氨酸磷酸酶1B 肿瘤坏死因子Α 罗格列酮 3T3-L1前脂肪细胞 脂肪细胞分化 

分 类 号：R346[医药卫生—基础医学]