枇杷叶中熊果酸的超声提取研究  

Study on the Extraction of Ursolic Acid from Loquat Leaf Via Ultrasonic Wave

作　　者：李开泉[1] 兰平生[2] 

机构地区：[1]宜春学院化学与生物工程学院,江西宜春336000 [2]丰城市中医院,江西丰城331100

出　　处：《中国现代应用药学》2007年第3期201-202,共2页Chinese Journal of Modern Applied Pharmacy

基　　金：江西省教育厅科研计划项目[赣财教字(2004)69号]

摘　　要：目的用超声波辅助提取法考察不同处理方法对熊果酸的提取效果。方法将枇杷叶及不同方法处理后的药渣,以乙醇为介质,用超声波辅助提取;同时用HPLC测定提取液中熊果酸的含量。结果枇杷叶中熊果酸的含量为0.9%以上,不同方法处理后的药渣中熊果酸均有不同程度的残留。结论熊果酸在水和油中几乎不溶;乙醇回流法的提取效果欠理想,增加提取次数,其得率无明显提高;枇杷叶中的熊果酸主要以游离形式存在。OBJECTIVE To investigate the effects of different treatment methods on extracting ursolic acid from Loquat Leaf with the aid of ultrasonic wave. METHODS After the precipitates obtained by the treatment of Loquat Leaf with different methods, ursolic acid is extracted by using ethanol as a medium under the ultrasonic wave. The content of ursolic acid is determined with HPLC method. RESULTS The content of ursolic acid in Loquat Leaf is above 0.9%. Some of ursolic acids remain in the precipitates after the different treatments. CONCLUSION Ursolic acid is almost indissoluble in water or oil ; the effect of ethanol refluxing extraction is not satisfactory, even increase the extract cycle, the yield of ursolic acid will not enhance obviously; Ursolic acid in Loquat Leaf exists mainly in dissociative form.

关 键 词：超声波 热回流 枇杷叶 熊果酸 提取效果 

分 类 号：R284.2[医药卫生—中药学]