地奥心血康对犬心肌缺血再灌注的血液过氧化脂质、氧自由基及红细胞超氧化物歧化酶活力的影响  

Effects of Di-ao-xin-xue-kang on serum lipid peroxide, coronary blood oxygen radical and superoxide dismutase activity of erythrocytes in myocardial reperfusion injury dogs

作　　者：赵丹[1] 李杨[1] 张键[1] 王蔚东[1] 赵雪俭[1] 刘忠荣[2] 周正质[2] 詹瑞云[3] 徐杰[3] 刘桂珍[3] 

机构地区：[1]白求恩医科大学病理生理教研室 [2]中国科学院成都地奥制药公司 [3]中国科学院长春应用化学研究所

出　　处：《新药与临床》1997年第1期12-14,共3页

摘　　要：目的：观察地奥心血康（ＤＡＸＸＫ）对犬心肌缺血再灌注的血液ＬＰＯ，氧自由基及红细胞ＳＯＤ的影响。方法：１８只成年杂种犬复制心肌缺血再灌注模型，分为ＤＡＸＸＫ治疗组和０．９％氯化钠注射液对照组。以荧光法测定血液ＬＰＯ；电子自旋共振（ＥＳＲ）行全血氧自由基捕捉；ＮＢＴ比色法测定红细胞及心肌中ＳＯＤ活力。结果：ＤＡＸＸＫ组血中ＬＰＯ含量低于对照组（Ｐ＜０．０５）；红细胞及心肌ＳＯＤ活力高于对照组（Ｐ＜０．０５，Ｐ＜０．０１）；对照组冠脉血中有强的氧自由基信号出现，而ＤＡＸＸＫ组的ＥＳＲ谱中氧自由基信号消失。结论：ＤＡＸＸＫ有降低心肌缺血再灌犬血中ＬＰＯ、氧自由基、提高红细胞及心肌ＳＯＤ活力的作用。AIM: To observe the effects of Di-ao-xin-xue-kang (DAXXK) on serum lipid peroxide (LPO), coronary blood oxygen radical, and superoxide dismutase (SOD) activity of erythrocytes in myocardial reperfusion injury dogs. METHODS: Mangrel dogs in myocardial ischemia reperfusion were divided into DAXXK group and 0.9% sodium chloride control group. Serum LPO contents were assayed by fluorescence method. Blood oxygen radicals were trapped by electron spin resonance (ESR) determination. SOD activities in erythrocytes and myocardia were determined by NBT colorimetric method. RESULTS: At 240 min after reperfusion, serum LPO contents were lower in DAXXK group than those in control group (P<0.05), SOD activities in erythrocytes and myocardia were significantly higher in DAXXK group than those in control group (P<0.05, P<0.01). Strong oxygen radical signals in coronary blood appeared in control group, while oxygen radical signals of ESR spectrum in DAXXK group disapeared. CONCLUSION: DAXXK decreases oxygen radicals and contents of serum LPO; increases SOD activities of erythrocytes and myocardia in myocardial ischemia reperfusion dogs.

关 键 词：地奥心血康 皂甙类 心肌缺血 药物疗法 

分 类 号：R541.405[医药卫生—心血管疾病]