低频脉冲磁场对带质粒大肠杆菌生化特性及质粒DNA的影响  

INFLUENCE OF LOW-FREQUENCY-MAGNETIC PULSE FIELDS ON BLOCHEMICALCHARACTERISTICS AND PLASMID DNA OF ESCHERICHIA COLI

作　　者：席晓莉[1] 范家骏[1] 于文彬[1] 杨春智[1] 饶国洲 苏明权 

机构地区：[1]第四军医大学生物医学工程系物理教研室,西京医院临床分子生物学教研室

出　　处：《中国医学物理学杂志》1997年第2期71-72,68,共3页Chinese Journal of Medical Physics

摘　　要：本文用频卒１５Ｈｚ、场强１５０ｍＴ的脉冲磁场对携带ｍｄｍ２ＣＤＮＡ片段的重组ｐＵＣ１８质粒，和携带ｐ５３ＣＤＮＡ片段的重组ｐＵＣ１８质粒的大肠杆菌（ＨＢ１０１）分别进行６小时和１２小时的辐照，检测其染色性及临床生化指标，并提取辐照组及对照组的质粒，琼脂糖电泳进行比较。结果表明，辐照６小时组的细菌约２０％的革兰氏染色性发生了改变，辐照１２小时组约３５％革兰氏染色性发生了改变，生化检测中，靛基质试验结果由阳性转变为阴性，麦芽糖和蔗糖虽为阳性，但与对照组相比显色有明显改变，琼脂糖电冰结果显示辐村组与对照组质粒无明显差异。提示低频脉冲磁场辐照可能影响大肠杆菌中某些酶的活性及代谢。: The Escherichia coli HB101 with recombitnation pUC18 plasmid with mdm2 CDNA fragment and p53 CDNA fragment were exposed to 15Hz 、1 50mT magnetic pulse fields for 6hr and 12hr respectivly. Their char- acteristics of Gram dyeability and biochemistry were observed , plasmids werc extracted and determined by elec- trophorisis. The results show that about 20% Gram dyeability of bacteria has changed after exposed to the mag- netic pulse fields for 6hr, while about 35% Gram dyeability of bacteria has changed after exposed to the magnetic fields for 12hr. The results of indal changed from positive to negative. Although maltose and sucrase trial show positive ,their dye were obviously tlifferent from the control groups. The electrophoresis bonds show that there aren't obviously difference between the trial groups and the control groups. The results indicate that the low-fre- quency-magnetic pulse fields may change the activity or metabolism of some enzymes in Escherichia coli.

关 键 词：磁场 生化特性 大肠杆菌 质粒DNA 生物电磁学 

分 类 号：Q64[生物学—生物物理学]