六味地黄方含药血清对氧化低密度脂蛋白损伤血管内皮细胞的保护作用  

Protective Effect of Rat Serum Containg Liuweidihuang Formula on Vascular Endothelial Cells from Oxidative Injury by Oxidized Low Density Lipoprotein

作　　者：张启春[1] 卞慧敏[1] 喻丽珍[1] 俞晶华[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《中国实验方剂学杂志》2007年第7期31-34,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省中医药局课题(H2004);江苏省教育厅自然科学基金(02KJB360005)

摘　　要：目的:观察六味地黄方大鼠含药血清对氧化低密度脂蛋白(ox-LDL)损伤血管内皮细胞(VEC)的保护作用。方法:以MTT法观察人脐静脉内皮细胞(HUVEC)的活力和流式细胞仪测定内皮细胞凋亡率,并通过测定胞内丙二醛(MDA)含量及细胞培养液中乳酸脱氢酶(LDH)、一氧化氮(NO)含量与超氧化物歧化酶(SOD)活力,探讨六味地黄方对血管内皮细胞损伤的保护作用及机制。结果:六味地黄方含药血清能促进ox LDL损伤的血管内皮细胞增殖、抑制ox LDL造成的内皮细胞凋亡、降低胞内MDA含量、减少细胞LDH释放量,提高SOD活力及NO含量。结论:六味地黄方对氧化低密度脂蛋白损伤的血管内皮细胞有一定的保护作用。Objective： To investigate the protective effects of Liuweidihuang Formula （LWDHF） on human umbilical vascular endothelial cells（HUVEC） injured by oxidized LDL（ox-LDL）. Methods：By assaying the proliferation ability of HUVEC using MTT method and the HUVEC apoptosis are with flow cytometry, meanwhile determining the level of malondialdehyde （MDA） in vivo, the levels of lactate dehyolrogenase（LDH）, nitric oxide （NO） and the activity of superoxide dismutase（SOD） in vitro, tht~ protective effect and mechanism of serum with LWDHF on injured HUVEC was assessed. Results： The serum with LWDHF increases the proliferation of HUVEC injured by ox-LDL and inhibits the apoptosis rate. It was also observed that the serum decreases the level of MDA, LDH and enhances the activity of SOD and the level of NO. Conclusion： LWDHF could prevent vascular endothelial cells from oxidative injury induced by ox- LDL.

关 键 词：六味地黄方 人脐静脉内皮细胞 氧化低密度脂蛋白 丙二醛 过氧化物歧化酶 乳酸脱氢酶 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]