化痰活血方拆方对高脂血症大鼠肝脏高密度脂蛋白受体基因表达的影响  

Influences of Divided Formula for Resolving Phlegm and Promoting Blood Circulation on the Expression of Scavenger Receptor,Class B Type Ⅰ(SR-BⅠ) in the Liver of the Hyperlipemia-modeled Rats

作　　者：闻莉[1] 刘松林[1] 叶勇[2] 梅国强[1] 

机构地区：[1]湖北中医学院,湖北武汉430061 [2]江汉大学医学与生命科学学院,湖北武汉430056

出　　处：《中国实验方剂学杂志》2007年第7期35-38,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖北省自然科学基金项目(2005ABA126);武汉市青年科技晨光计划项目(20055003059-35);湖北中医学院2005年科研立项课题(2005-11)

摘　　要：目的:拆方观察化痰活血方中化痰与活血药对高脂血症大鼠肝脏高密度脂蛋白受体(SR-BⅠ)基因表达的影响,从分子水平明确该方防治高脂血症的有效作用部位。方法:Wistar大鼠50只,随机分为空白组、模型组、全方组、化痰药组、活血药组,采用高脂饲料建立大鼠高脂血症模型。连续灌胃给药30 d后,应用逆转录聚合酶链式反应(RT-PCR)法测定各组大鼠肝脏SR-BImRNA水平。结果:模型组大鼠肝SR-BImRNA表达明显低于空白组,化痰活血全方组和化痰药组大鼠肝SR-BImRNA水平明显高于模型组,而活血药组和模型组无明显差异,结论:化痰药是化痰活血方中促进高脂血症模型大鼠肝脏SR-BImRNA水平表达的主要组分,因而也是调节脂质代谢的主要作用部位。Objective： To observe the influences divided formula for resolving phlegm and promoting blood circulation on the expression of scavenger receptor, class B type Ⅰ （SR-B Ⅰ ） in the liver of the hyperlipemia-modeled rats. Methods：50 Wistar rats were divided randomly into 5 groups：blank group, model group, full formula group, group of taking drugs for resolving phlegm, and group of taking drugs for promoting blood circulation. The model was established by high fat feeding into stomach daily except the blank group. After 30 days RT-PCR was adopted to messure the level of SR-B Ⅰ mRNA for each group. Results： The level of SR-B I mRNA of model group was apparently lower than that of blank group. Compared to that of the model, SR-B I mRNA of the full formula group and the group of taking drugs for resolving phlegm increased evidently, but there was no difference between model group and the group of taking drugs for promoting blood circulation for SR-B Ⅰ mRNA. Conclusion： In the full formula, the drugs for resolving phlegm play the most important role in elevating the level of SR-B Ⅰ mRNA in the liver of the hyperlipemia-modeled rats.

关 键 词：高脂血症 化痰活血方 化痰药 活血药 高密度脂蛋白受体 

分 类 号：R285.5[医药卫生—中药学]