苏太猪SLA-DQB基因外显子2多态性及其与繁殖性能的关联分析  

Polymorphism of the Exon2 of SLA-DQB Gene and Its Relationship with Reproductive Performance in Sutai Pigs

作　　者：包文斌[1] 吴圣龙[1] 陈国宏[1] 鞠慧萍[1] 华金第 黄雪根 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省苏州市苏太猪育种中心,苏州215128

出　　处：《畜牧兽医学报》2007年第7期636-640,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省高技术研究(农业)(BG2006302)

摘　　要：采用PCR-RFLP法对苏太猪SLA-DQB基因外显子2的PCR产物进行分析,结果显示,SLA-DQB基因外显子2经RsaⅠ酶切后,共分出4种基因型;经HaeⅢ酶切,共分出3种基因型。χ2适合性检验结果表明,苏太猪SLA-DQB基因外显子2在RsaⅠ和HaeⅢ酶切位点都已经达到了Hardy-Weinberg平衡状态。在RsaⅠ和HaeⅢ酶切后分出的4种组合带型中,组合带型为BBEE的种母猪第3胎总产仔数、产活仔数、初生窝重、断奶仔猪数和断奶窝重均显著高于组合带型为AADD的个体(P<0.05),也高于组合带型为ABDE和ACDE的个体,但是差异不显著(P>0.05)。The exon 2 of SLA-DQB gene in Sutai pigs was analyzed by PCR-RFLP method, the results showed that the exon 2 of SLA-DQB gene digested by restriction endonuclease Rsa Ⅰ and Hae Ⅲ could be divided into four kinds of genotypes and three kinds of genotypes in Sutai pigs respectively. The results of χ^2 indicated that the frequencies of patterns digested by Rsa Ⅰ and Hae Ⅲ fit with Hardy-Weinberg equilibrium in Sutai population. Four kinds of combined patterns were observed in Sutai pigs. The correlation between these combined patterns and the reproductive performances of Sutai pigs was analyzed, the results showed that reproductive performances （the total litter, number of viable pigs, litter weight at birth, number of ablactation pigs and litter weight after ablactation in the third litter） of Sutai pigs with BBEE combined pattern were significant higher than those with AADD combined pattern, also higher than the pigs with ABDE combined pattern and ACDE combined pattern, but were no significant difference in reproductive performances between pigs with AADD combined pattern and the pigs with ABDE and ACDE combined patterns.

关 键 词：猪 SLA-DQB PCR-RFLP 

分 类 号：S828.2[农业科学—畜牧学]