Artificial induction of androgenetic diploid in Yellow catfish(Pelteobagrus fulvidraco)  

作　　者：XU Hui FAN Zhaoting CHEN Weixing FANG Jingjie 

机构地区：[1]College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2007年第2期110-114,共5页东北农业大学学报（英文版）

基　　金：Supported by Science Project of Heilongjiang Province(GC03B510)

摘　　要：Androgenesis was the especial zoogamy that the germ plasma of offspring was from the agnate. In this study the eggs of Yellow catfish （Pelteobagrus fulvidraco） were irradiated by UV suspending in the synthetic ovarian fluid （OF）, and the total dosage of UV irradiation was 220 mJ·cm^-2. Diploid could be induced by heat shock （40℃, 2 min, 3 min） with different time period after fertilization （15-37 min）. The result showed that heat shock with 2 min was better than 3 min; there were two apices of induction in 17-21 min and 27-31 min after fertilization. The highest hatching rate was 3.30% at 29 min after fertilization, and the difference between two apices of induction was unobvious.Androgenesis was the especial zoogamy that the germ plasma of offspring was from the agnate. In this study the eggs of Yellow catfish （Pelteobagrus fulvidraco） were irradiated by UV suspending in the synthetic ovarian fluid （OF）, and the total dosage of UV irradiation was 220 mJ·cm^-2. Diploid could be induced by heat shock （40℃, 2 min, 3 min） with different time period after fertilization （15-37 min）. The result showed that heat shock with 2 min was better than 3 min; there were two apices of induction in 17-21 min and 27-31 min after fertilization. The highest hatching rate was 3.30% at 29 min after fertilization, and the difference between two apices of induction was unobvious.

关 键 词：Pelteobagrus fulvidraco heat shock ANDROGENESIS DIPLOID 

分 类 号：S965.1[农业科学—水产养殖] Q813.4[农业科学—水产科学]