鳗鲡拟指环虫的免疫原性  

Immunogenicity of Pseudodactylogyrus spp. from eel,Anguilla sp.

作　　者：刘晓东[1] 李敏[2] 朱春华[3] 陈强[1] 林天龙[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003 [2]福建师范大学生命科学学院,福建福州350007 [3]福建农林大学动物科学学院,福建福州350002

出　　处：《中国水产科学》2007年第4期654-658,共5页Journal of Fishery Sciences of China

基　　金：福建省科技重大专项(2004NZ03-2);福建省农业科学院院长基金(2005256预-13)

摘　　要：拟指环虫病是严重危害养殖鳗鲡(Anguilla sp.)的寄生虫病,为了寻求更为安全有效的免疫防治方法,对鳗鲡拟指环虫(Pseudodactylogyrus spp.)的结构蛋白及其免疫原性进行分析。以鳗鲡拟指环虫全虫蛋白为抗原,制备了鼠和鳗鲡抗拟指环虫免疫血清,ELISA检测效价分别为1∶51 200和1∶3 200。SDS-PAGE分析表明,虫体含有16 kD、21 kD、29 kD、37 kD、43.5 kD6、8 kD和110 kD等主要结构蛋白,其中29 kD的多肽为高丰度蛋白;Western blot证实免疫鳗和康复鳗血清均能识别25 kD、43.5 kD6、2 kD、81 kD等虫体蛋白。间接荧光抗体试验显示,个别虫体在头部、尾部有明显的荧光染色,大多数虫体中部两侧,特别是卵黄腺丰富区域,呈较强的荧光染色。Pseudodactylogyrosis infection is a severe disease that threatens the eel industry. Due to the repeated and persistent infection of Pseudodactylogyrosis, the anthelmintics were frequently applied to control the branchial parasite Pseudodactylogyrus spp. in eel, which caused the Pseudodactylogyrus extensively resisting to many anthelmintics, and made the prophylaxes and therapy of Pseudodactylogyrosis became a thorny matter. In the present study,analysis of the structural protein and immunogenicity of Pseudodacty-logyrus is described. The purpose of the study was to seek a safer and more effective approach to resist Pseudodactylogyrosis by means of immunological technique. The mouse and eel antisera were prepared with sonicated Pseudodactylogyrus as immunogen. The antibody titers of mouse and eel antisera tested by ELISA were 1 ： 51 200 and 1 ： 3 200, respectively. SDS-PAGE analysis of Pseudodactylogyrus proteins was performed, and then stained with silver nitrate. The results showed that sonicated Pseudodactylogyrus contained seven major structural proteins with molecular weight of 16 kD, 21 kD, 29 kD, 37 kD, 43.5 kD, 68 kD and 110 kD, respectively, among which the 29 kD polypeptide was most abundant. The sera of both immunized eel and convalescent eel recognized the 25 kD,43.5 kD,62 kD and 81 kD proteins of sonicated Pseudo-dactylogyrus on Western blot. Significantly elevated anti-Pseuctodactylogyrus antibodies were detected in the immunized fish. The immunofluorescence staining revealed that, in addition to some bright fluorescence spot being observed on the head and tail of individual Pseudodactylogyrus, both sides in the middle part of Pseucloclactylogyrus ,especially the areas which was adjacent to yolk gland showed stronger fluorescence. [Journal of Fishery Sciences of China, 2007,14 （4）：654- 658 ]

关 键 词：鳗鲡 拟指环虫 结构蛋白 免疫原性 

分 类 号：S941.5[农业科学—水产养殖]