慢性肺原性心脏病急性加重期血浆内皮素-1与血小板功能关系的探讨  

Relationship between plasma endothelin and platelet aggregation and cytosolic free calcium levels in chronic cor pulmonale/

作　　者：马中富[1,2] 李天木[1,2] 蒋祖勋[1,2] 关开泮[1,2] 徐钟源[1,2] 詹红[1,2] 

机构地区：[1]中山医科大学附一院急诊科 [2]广东省人民医院心研所

出　　处：《中国危重病急救医学》1997年第4期216-218,共3页Chinese Critical Care Medicine

摘　　要：目的：探讨血浆内皮素１（ＥＴ１）、血小板聚集及血小板内游离Ｃａ２＋在慢性肺原性心脏病（肺心病）急性加重期的意义及其相互关系。方法：用放射免疫分析法测定两组（４２例慢性肺心病患者急性加重期和２５例健康者）血浆ＥＴ１含量，用比浊法测定两组血小板聚集率和用Ｆｕｒａ２／ＡＭ荧光技术测定血小板内游离Ｃａ２＋含量。结果：慢性肺心病急性加重期ＥＴ１水平明显升高（Ｐ＜０．０１），血小板聚集率增高（Ｐ＜０．０１），血小板内游离Ｃａ２＋含量显著增加（Ｐ＜０．０１）。另外还观察到ＡＤＰ诱导的血小板聚集率与血小板内游离Ｃａ２＋含量之间呈正相关（ｒ＝０．７３８，Ｐ＜０．０１），而血浆ＥＴ１水平与血小板聚集率和血小板内游离Ｃａ２＋含量之间无明显相关性。结论：血浆ＥＴ１在慢性肺心病急性加重期的发病中可能起一定作用。此期血小板的功能处于活跃状态，易于聚集，形成血栓，加重病情；而血浆ＥＴ１对血小板功能可能无影响。Objective:To investigate the relationship between plasma endothelin1(ET1) and platelet aggregation and cytosolic free calcium levels in patients with chro nic cor pulmonale.Methods:Plasma ET1 level was measured by radioimmunoassay (RIA) in 42 patients with chronic cor pulmonale during the acute phase and 25 healthy volunteers.Meanwhile,platelet aggregation induced by ADP was measured with LmiAggreometer M 450 ,and cytosolic free calcium level in Fura2 loaded platelet.Results:It was shown that plasma levels of ET1 and platelet aggregation induced by ADP were significantly higher in patients than those in controls,so did the cytosolic free calcium levels.There was a positive relationship between the platelet aggregation induced by 5μmol/L ADP and free calcium levels ( r =0.738, P <0.01) in patients,but no correlation between plasma ET1 levels and platelet aggregation or free calcium levels was found.Conclusions:It is suggested that plasma ET1 may play an important role in the pathogenesis of chronic cor pulmonale,but does not affect the function of activated platelet.

关 键 词：内皮素 血小板功能 肺心病 病理 

分 类 号：R541.502[医药卫生—心血管疾病]