板栗疫病菌低毒力遗传转化株LC的构建  

Construction of engineered hypovirulent Cryphonectria parasitica strain LC

作　　者：蓝秀万[1] 蓝瑛[1] 林海燕[1] 陈保善[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005

出　　处：《广西农业生物科学》2007年第2期106-109,174,共5页Journal of Guangxi Agricultural and Biological Science

基　　金：国家自然科学基金重点项目(30130020);国家杰出青年科学基金(39925003);教育部高校骨干教师资助计划(2000);广西自然科学基金(桂自科0229001)

摘　　要：低毒病毒—板栗疫病菌组合是研究病毒—宿主相互作用的模式系统之一。通过构建含板栗疫病菌低毒病毒CHV1-EP713的全长cDNA和苯菌灵抗性基因的表达质粒pXB3F2,并用于转化野生型无毒株EP155,获得了以苯菌灵抗性为筛选标记、具有dsRNA病毒再生能力的低毒力遗传转化株LC,为深入研究病毒—真核宿主相互作用的分子机制提供了新的研究材料。Hypovirus/chestnut blight fungus （Cryphonectria parasitica） is an excellent experimental system to study the virus-host interaction, pXBn3F2, a plasmid with benomyl resistance gene and the full-length cDNA of Cryphonectria parasitica hypovirus CHV1-EP713, was constructed and used to transform hypovirus-free C. parasitica strain EP155. A resultant transformant designated LC that exhibited benomyl resistance and harbored the resurrected hypovirus CHV1-713 dsRNA was obtained. The availability of the engineered strain LC provides a valuable material for studying the molecular mechanisms of the interaction between hypovirus and its eukaryotic host fungus.

关 键 词：板栗疫病菌 低毒病毒 低毒力遗传转化株LC CHV1-EP713 EP155 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]