甘蔗茎RNA提取方法的比较  

Comparison of sugarcane stem RNA extracting methods

作　　者：王爱勤[1] 范业赓[1] 赵晓艳[1] 何龙飞[1] 黄荣韶[1] 杨丽涛[1] 李杨瑞[2] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广西农业科学院作物遗传改良与生物技术重点实验室,广西南宁530007

出　　处：《广西农业生物科学》2007年第2期175-178,共4页Journal of Guangxi Agricultural and Biological Science

基　　金：广西科学基金项目(桂科青0640003);广西农科院博士后基金项目(52274);广西大学博士启动基金项目

摘　　要：以甘蔗茎为材料,分别用异硫氰酸胍法与Trizol提取法,比较从蔗汁、蔗渣和汁渣混合物匀浆中提取甘蔗茎总RNA的效果。结果表明,从蔗汁和汁渣混合物匀浆中提取到28S rRNA、18S rRNA和5S rRNA,而蔗渣中仅提取到5S RNA;蔗汁中提取的mRNA,其转录水平可代表甘蔗茎mRNA的转录水平;异硫氰酸胍法提取的蔗汁总RNA纯度和产量均优于Trizol提取法的。Total RNA of sugarcane stem was extracted from sugar juice, bagasse and mixture of juice and bagasse by using the methods of isothiocyanate and Trizol Reagent Kit. The results showed that 28S rRNA, 18S rRNA and 5S rRNA were extracted from sugar juice and mixture of juice and bagasse, and 5S RNA was extracted from bagasse only. Transcriptional level of mRNA extracted from sugar juice can stand for mRNA transcriptional level of sugarcane stem. Total RNA purity and yield from sugar juice extracted by isothiocyanate method was higher than that by Trizol Reagent Kit method.

关 键 词：甘蔗 茎 异硫氰酸胍 TRIZOL 

分 类 号：S566.1[农业科学—作物学]