乙肝肾患儿血清、肾脏和肝脏组织内乙肝病毒感染标志物的比较  

Comparison of HBV Markers in Serum,Kidney and Liver Tissue from Children with Hepatitis B Virus-Associated Glomerulonephritis

作　　者：黄爱文[1] 何庆南[1] 周频[1] 党西强[1] 莫双红[1] 易著文[1] 

机构地区：[1]中南大学湘雅二医院儿科湘雅二医院小儿肾脏病研究室,湖南长沙410011

出　　处：《现代生物医学进展》2007年第7期1045-1047,共3页Progress in Modern Biomedicine

摘　　要：目的:比较分析乙型肝炎病毒相关性肾炎(HBV-GN)患儿血清、肾脏和肝脏组织内HBV感染标志物的表达情况。方法:45例血清HBV感染标志物阳性的HBV-GN患儿经肾脏组织活检确诊,其中13例同时进行肝脏组织活检,且行HBV感染标志物免疫组织化学检查。结果:45例HBV-GN肾脏病理改变为:膜性肾病(MN)25例(55.6%)、系膜增生性肾炎(MsPGN)11例(24.4%)、膜增生性肾炎(MPGN)5例(11.1%)、毛细血管内皮增生性肾炎(EPGN)4例(8.9%),免疫荧光检查显示肾脏组织内以IgG(40例,88.9%)和C3(34例,75.6%)沉积为主。血清HBV感染标志物HBeAg(+)组25例(55.6%),HBeAg(-)组20例(44.4%);肾脏组织中HBcAg的阳性率为80.0%(36/45),其中,血清HBeAg(-)组肾脏组织HBcAg阳性率(100.0%)明显高于血清HBeAg(+)组(64.0%),(P=0.002)。肝肾同检结果显示HBV感染标志物(HBsAg和HBcAg)在肝脏组织表达的阳性率分别为84.6%和53.8%,在肾脏组织表达的阳性率分别为84.6%和76.9%,且肝、肾组织中HBV感染标志物表达不同步。结论:HBV-GN患儿血清、肾脏和肝脏组织内HBV感染标志物表达呈明显的不一致性。Objective： To compare with the presence of HBV markers in serum, renal and fiver tissues from children with hepatitis B virus-associated glomenflonephritis （HBV-GN）. Methods： 45 cases of HBV-GN were confirmed by renal biopsy, 13 of which were carried out fiver biopsy simultaneously. The biopsy tissues were studied by immunohistochemical stain for markers of HBV. Results： Renal histology in these 45 cases showed membranous nephropathy 25 cases （55.60/0）, mesangial proliferative glomerulonephritis 11 cases （24.4%）, membranoprofiferative glomerulonephritis 5 cases （ 11.1% ） and endocapillary proliferative glomerulonephritis 4 cases （8.9%）. Immunofluorescence showed mainly IgG（40 cases, 88.9%） and C3 deposit（34 cases, 75.6%） in renal tissue. Serum HbeAg（＋ ）group accounted for 55.6% （25 cases）, and HbeAg（-） group accounted for 44.4% （20 cases） in total 45 HBV-GN cases. HBcAg stain positive rate in renal tissue in serum HbeAg （-） group （100.0%） was significantly higher than that of HbeAg（＋） group （64.0%）, （P=0.002）. Meanwhile the expression of HBsAg and HBcAg in fiver and renal tissue is not parallel. Conclusion： The expression of HBV markers in serum, renal and fiver tissues in children with HBV-GN is not consistency.

关 键 词：肝炎病毒 乙型 肾炎 病理学 免疫组织化学 生物学标记 

分 类 号：R725.7[医药卫生—儿科]