松毛虫DpKettin基因片段的克隆与初步定位研究  

Cloning and mapping of DpKettin gene on Z chrmosome of the pine caterpillar by Real-time quantitative PCR

作　　者：牛宝龙[1] 翁宏飚[1] 何丽华[1] 沈卫锋[1] 孟智启[1] 

机构地区：[1]浙江省农业科学院蚕桑研究所,杭州310021

出　　处：《遗传》2007年第7期823-828,共6页Hereditas（Beijing)

基　　金：浙江省科技攻关项目(编号:2004C22037)~~

摘　　要：对于ZW型鳞翅目昆虫,雄性为ZZ,雌性为ZW;细胞内Z染色体数与常染色体组数之比在雌雄间存在差异,雄性为2Z∶2A=1.0,雌性为Z∶2A=0.5,如果某物种一个基因(假如K基因)位于Z染色体上,另一个基因(假如N基因)位于常染色体上,则雄体中2K∶2N=1.0,雌体中K∶2N=0.5。研究利用家蚕、棉铃虫等昆虫Kettin基因序列,克隆了松毛虫的同源基因(DpKettin)片段,并采用荧光定量PCR技术,以松毛虫的ANT基因为参照基因,检测松毛虫雌雄不同个体间DpKettin基因与腺苷酸转移酶基因(ANT)的拷贝数之比,结果表明:雄体DpKettin∶ANT=1.0,雌体DpKettin∶ANT=0.5,说明DpKettin基因位于松毛虫Z染色体上。The ratio of Z chromosome number to autosomal chromosome （A） is different between male and female lepidoptera insects. In males： 2Z ： 2A=1, and in female： Z ： 2A=0.5. The ratio of the copies of gene （such as K） on Z chromosome number to the copies of gene （such as N） on autosomal chromosome （K/N） is also different between male and female. In males： 2K ： 2N =1, and in female： K ： 2N=0.5. The DpKettin gene of the pine caterpillar Dendrolimus punctatus was cloned with the primers designed according to the sequences of the BmKettin of the silkworm and the HaKettin of the cotton bollworm. The ratio of copies between DpKettin and ANT （adenine nucleotide translocator） in male and female was deter- mined by the real-time quantitative PCR technique. They were 1.0 in males and 0.5 in females. These ratios were equal to the ratios of the copies of gene on Z chromosome number to the copies of gene on autosomal chromosome. It indicates that DpKettin is located on Z chromosome in the genome of the pine caterpillar.

关 键 词：DpKettin基因 ANT基因 荧光定量PCR Z染色体 松毛虫 

分 类 号：S763.3[农业科学—森林保护学]