粘质沙雷氏菌几丁质酶chiB基因的克隆与序列分析(英文)  

Cloning and Sequence Analysis of Serratia marcescens Chitinase chiB Gene

作　　者：叶辉[1] 程备久[2] 朱苏文[2] 甘德芳[3] 冯春[4] 

机构地区：[1]安徽农业大学生物技术中心,安徽合肥230036 [2]安徽农业大学生命科学学院,安徽合肥230036 [3]安徽农业大学园艺学院,安徽合肥230036 [4]安徽农业大学农业因子试验总场管理中心,安徽合肥230036

出　　处：《激光生物学报》2007年第3期299-303,共5页Acta Laser Biology Sinica

基　　金：Scientifical Item of Education Department of Anhui Province(No.2006KJ213B)

摘　　要：采用改进的方法提取粘质沙雷氏菌基因组DNA,通过PCR扩增,得到大小为1 500 bp特异性DNA片段(chiB基因),以pUC18质粒构建了pUC-ch iB克隆载体,转化至感受态细胞E.coliDH5α培养,并筛选出重组质粒。经测序分析,证明克隆片段与文献报道相一致。The genome DNA was extracted from Serratia marcescens by improved method. A special fragment about 1 500 bp length was cloned from Serratia marcescens genome DNA by Polymerase Chain Reaction（PCR） amplification. Vector pUC-chiB was constructed through ligating the fragment into the plasmid pUC18 and transformed into E. coli DH5α. Through screening of recombinants and sequence analysis of it, the result showed that the cloned DNA fragment was chitinase chiB gene of Serratia marcescens which was the same as reported.

关 键 词：几丁质酶 chiB基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]