不同性别类型苦瓜基因组DNA提取及RAPD标记初步研究  

Effective extraction of genomic DNA and RAPD analysis on different sex types of Momordica charantia L.

作　　者：邓俭英[1] 王绪[2] 方锋学[1] 张曼[1] 孙德利[2] 

机构地区：[1]广西农业科学院蔬菜研究中心,南宁530007 [2]广西大学,南宁530004

出　　处：《广西农业科学》2007年第3期223-226,共4页Guangxi Agricultural Sciences

基　　金：广西"新世纪十百千人才工程"专项(2003211);广西青年科学基金项目(桂科青0447015);广西农科院科技发展基金项目[2003006Z]

摘　　要：采用改良的CTAB法成功地提取了不同性别类型苦瓜品种的基因组DNA,作为PCR扩增反应模板进行RAPD分析。结果表明,从200个10bp随机寡核苷酸引物中筛选到2个引物S30和S66,其可以在全雌性品种X-黑-d-d稳定地扩增出特异性条带,为下一步克隆苦瓜性别决定基因、探索苦瓜性别决定机理提供了试验依据。至于该特异性标记是否与苦瓜雌性性别有关,还有待进一步分析验证。The genomic DNA was effectively extracted from different sex types of Momordica charantia L. by modified CTAB method, and employed as the template for PCR amplification reaction in RAPD analysis. A total of 200 arbitrary 10-meroligonucleotide primers were screened. Among them, two primers of S30 and S66 produced specific band in gynoecious cultivar X-Hei-d-d. The results provided the basis on cloning the sty gene of Momordica charantia L. andstudying the mechanism of sex-determining gene. Moreover, further studies on the relationship between specific band and female sex of Momordica charantia L. are expected.

关 键 词：苦瓜 性别类型 基因组DNA RAPD标记 

分 类 号：S642.5[农业科学—蔬菜学]