金黄色葡萄球菌血清5型生长曲线的测定  

Determination of Growth Curve of Serotype 5 of Staphylococcus aureus

作　　者：刘庆涛[1] 李颖[1] 杨正涛[1] 张乃生[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《安徽农业科学》2007年第19期5662-5663,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金"双特异性抗体的制备及治疗奶牛金葡菌性乳腺炎的作用机制"的一部分(C02030603)

摘　　要：[目的]为了测定金黄色葡萄球菌血清5型的生长曲线。[方法]在不同培养基和不同培养方式下,用分光光度计定时测定菌液吸收值,绘制了金黄色葡萄球菌的生长曲线。[结果]在哥伦比亚培养基中,液体培养基在振荡条件下比固体培养基更有利于金黄色葡萄球菌的生长,在静止条件下最不利于金黄色葡萄球菌的生长;在Mod110培养基中,金黄色葡萄球菌的生长速度为固体液体(振荡)液体(静止)。在相同培养方式下,Mod110固体培养基比哥伦比亚固体培养基更有利于金黄色葡萄球菌的生长。[结论]该生长曲线反映了细菌的生长趋势,说明细菌可以进行较长时间的研究。[Objective] The study was conducted to determine the growth curve of serotype 5 of Staphylococcus aureus.[Method] With different mediums and culture modes,the absorption value of bacterium fluid was determined with spectrophotometer,and the growth curve of Staphylococcus aureus was drawn.[Result] In Columbia culture medium,the liquid medium more favored to the growth of Staphylococcus aureus than solid medium under shaking condition,and it was worst under stationary condition.In Mod 110 culture medium,the growing speed of Staphylococcus aureus was as follows： solid〉liquid （shaking condition） 〉 liquid （stationary condition）. With the same culture pattem, Mod 110 medium was better than Columbia medium to the growth of Staphylococcus aureus. [Conclusion] The growth curve reflected growing trend of the bacterium, indicating that the bacterium could be researched for long term.

关 键 词：金黄色葡萄球菌 血清5型 生长曲线 

分 类 号：S852.6[农业科学—基础兽医学]