bFGF对弥漫性轴突损伤大鼠病理及免疫组化改变的影响  

Effects of bFGF on alterations in pathology and immunohistochemistry in rats with diffuse axonal injury

作　　者：文立[1] 戴炯[1] 胡威夷[2] 李善泉[1] 

机构地区：[1]上海交通大学医学院仁济医院神经外科,上海200001 [2]中国人民解放军成都军区总医院神经外科,成都610083

出　　处：《上海交通大学学报（医学版）》2007年第3期256-259,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的研究弥漫性轴突损伤(DAI)大鼠病理及免疫组化改变以及碱性成纤维细胞生长因子(bFGF)对其影响。方法采用重物撞击法致伤大鼠。bFGF治疗组(n=60)大鼠硬膜下及皮层下注射bFGF,另设正常对照组(n=20)和致伤对照组(n=60)。光镜和电镜下观察病理改变;免疫组化检测脑源性神经营养因子(BDNF)、生长相关蛋白-43(GAP-43)和胶质纤维酸性蛋白(GFAP)的表达。结果大鼠DAI后,桥脑基底部、胼胝体区及大脑半球白质呈现典型病理改变,并有GFAP、GAP-43及BDNF表达升高。bFGF治疗组病理改变较轻。bFGF能促进BDNF和GAP-43表达,抑制GFAP表达。结论大鼠DAI后,局灶应用bFGF能促进脑组织再生,加速功能恢复,但其长期疗效尚不确定。Objective To study the alterations in pathology and immunohistochemistry in rats with diffuse axonal injury （DAI） and the effects from basic fibroblast growth factor （ bFGF）. Methods A weight-drop device was employed to produce DAI in rats. In the treatment group （n = 60） , bFGF was injected subdurally and subcortically. Besides, normal control group （n = 20） and injury-control group （n =60） were also established. The pathological changes were observed by light microscopy and electromicroscopy, and the expression of brain-derived neurotrophic factor （BDNF）, growth associated protein-43 （GAP-43） and glial fibrillary acidic protein （GFAP） were also examined by immunohistochemistry. Results Typical pathological changes were observed in the basal portion of pons, corpus callosum and white matter of cerebral hemisphere in the rats with DAI. And an upregulation of GFAP, GAP-43 and BDNF was also found. In the treatment group, better outcomes of pathological changes were observed, bFGF increased the expression of BDNF and GAP-43, while inhibited the immunoreactivity of GFAP. Conclusion Topical application of bFGF can improve brain tissue regeneration and speed function recovery in rats with DAI, though its long-term effect warrants further study.

关 键 词：弥漫性轴突损伤 碱性成纤维细胞生长因子 脑源性神经营养因子 生长相关蛋白-43 胶质纤维酸性蛋白 大鼠 

分 类 号：R651.1[医药卫生—外科学]