bFGF对弥漫性轴突损伤大鼠认知能力的影响  

Effects of bFGF on cognitive ability of rat with diffuse axonal injury

作　　者：文立[1] 戴炯[1] 胡威夷[2] 李善泉[1] 

机构地区：[1]上海交通大学医学院仁济医院神经外科,上海200001 [2]中国人民解放军成都军区总医院神经外科,成都610083

出　　处：《上海交通大学学报（医学版）》2007年第3期260-264,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的研究弥漫性轴突损伤(DAI)大鼠的神经行为学改变及碱性成纤维细胞生长因子(bFGF)对其的影响。方法采用重物撞击法致伤大鼠。bFGF治疗组(n=60)大鼠硬膜下及皮层下注射bFGF,另设正常对照组(n=20)和致伤对照组(n=60)。用感觉运动整合试验、高杆爬行试验、抓握悬吊试验和水迷宫试验检测大鼠运动和学习记忆能力。颅骨内电极植入术后行类P3检测。结果大鼠DAI后感觉运动整合试验、高杆爬行试验、抓握悬吊试验及水迷宫试验成绩均有不同程度下降,bFGF治疗组早期成绩较佳。大鼠DAI后P3 a潜伏期延长,致伤对照组P3 a潜伏期长于bFGF治疗组(P<0.05)。结论大鼠DAI后学习记忆和运动能力下降,伴类P3潜伏期延长;事件相关电位(ERP)有希望用于DAI监测;bFGF脑内局灶应用能减轻大鼠DAI。Objective To study the alterations in neurobehavior of rats with diffuse axonal injury（DAI） and to evaluate the potential role of basic fibroblast growth factor （bFGF）. Methods A weight-drop device was employed to build the DAI model. An injection of bFGF subdurally and subcortically was given to the bFGF therapeutic group（n = 60）. Besides, normal control （n =20） and injured control group （n =60） were established. The elevated walkway test, prehensile traction test, sensorimotor integration test and the Morris＇ water maze test were adopted to examine the motor and memory abilities. After the implantation of skull electrodes, P3-like potential was explored in rats before and after injury. Results After DAI, the scales of the elevated walkway test, prehensile traction test, sensorimotor integration test and the Morris＇ water maze test were decreased, and the rats in the bFGF therapeutic group presented a better behaviour in the early stage. The latency of P3-1ike potential prolonged significantly in rats with DAI,with the P3-1ike potential in the injured control group longer than that in the bFGF therapeutic group （P 〈 0.05）. Conclusion After DAI, motor and memory dysfunction with a prolonged latency of P3-1ike potential can be found in rats. Event-related potential may be helpful in the monitor of DAI. Neurobehavioral damages in rats with DAI can be improved by topically application of bFGF.

关 键 词：弥漫性轴突损伤 碱性成纤维细胞生长因子 神经行为 事件相关电位 大鼠 

分 类 号：R651.1[医药卫生—外科学]