丹参酮Ⅱ-A在RAW264.7细胞系中的抗炎症作用机制  

Anti-Inflammatory Mechanism of the Important Ingredient of Salvia miltiorrhiza Bunge-Tanshinone Ⅱ-A in RAW264.7 Cell Line

作　　者：唐涛[1] 郭伟强[1] 王珏[1] 陈明洁[1] 何光源[1] 

机构地区：[1]华中科技大学中英HUST-RRes作物基因工程和基因组学联合实验室,湖北武汉430073

出　　处：《生物技术通讯》2007年第1期51-53,共3页Letters in Biotechnology

基　　金：国家重点基础研究发展计划项目(2002CB111302);国家自然科学基金项目(30370807)

摘　　要：目的:丹参酮Ⅱ-A是丹参的一种重要成分。研究丹参酮Ⅱ-A的抗炎症机制。方法:以小鼠腹腔巨噬细胞系RAW264.7作为药物刺激靶细胞,使用不同浓度的分析纯丹参酮Ⅱ-A对RAW264.7细胞系进行刺激,在细胞被刺激24、48h后,使用MTT比色法和半定量RT-PCR法对刺激后的细胞进行检测。结果:通过MTT比色法检测,发现丹参酮Ⅱ-A抑制炎症细胞的增殖,初步计算出丹参酮Ⅱ-A的半抑制浓度(IC50)为43.2μmol/L;在半定量RT-PCR实验中发现,在发生炎症后,丹参酮Ⅱ-A通过抑制磷脂酶A2来减轻炎症。结论:在炎症发生后,丹参酮Ⅱ-A通过抑制磷脂酶A2来达到其抗炎症的作用。Objective： To research the anti-inflammatory mechanism of tanshinoneⅡ-A, which is an important ingredient in Salvia miltiorrhiza Bunge. Methods： RAW264.7 cell lines were inhibited by tanshinone Ⅱ-A with different concentration as target cell. When RAW264.7 were inhibited for 24, 48 h, used MTT assay and semi-quantitative RT-PCR. Results： According to MTT assay, IC50 of the anti-inflammatory is 43.2 μmol/L; in semi-quantitative RT-PCR experiment, tanshinone Ⅱ-A inhibit the action of phospholipases A2. Conclusion： The results also demonstrate the action of anti-inflammatory mechanism is performed by inhibiting the action of phospholipases A2.

关 键 词：丹参酮Ⅱ-A MTT比色法 半定量RT-PCR 磷脂酶A2 

分 类 号：Q946[生物学—植物学] R285[医药卫生—中药学]