机械振荡对猕猴桃试管苗根系发育的影响  

Effects of Mechanical Vibration on Roots Development of Actinidia chinensis Plantlet

作　　者：阳小成[1,2] 王伯初[3] 叶梅[4] 

机构地区：[1]成都理工大学材料与化学化工学院 [2]重庆大学生物工程学院生物力学与组织工程教育部重点实验室,重庆400044 [3]重庆大学生物工程学院生物力学与组织工程教育部重点实验室 [4]重庆工商大学环境与生物工程学院

出　　处：《生物技术通讯》2007年第1期81-83,共3页Letters in Biotechnology

基　　金：国家自然科学基金项目(39770206)

摘　　要：目的:研究外界环境应力刺激对木本植物幼苗根系发育的生物学效应。方法:通过模拟机械振荡刺激中华猕猴桃的试管苗。结果:适度的机械振荡(频率2～3 Hz)可促进猕猴桃试管苗根系的生长发育,根系质膜的通透性降低,根尖活力以及根系总长、分根数等指标明显增加;但当振动强度超过一定值(4Hz)后,根系活力的增强效应就受到明显的抑制。结论:植物根系发育对外界应力刺激有较显著的双向效应;从细胞和分子生物学水平探讨了环境应力影响试管苗根系发育可能的内在机制。Objective： The purpose of this study is probing into the relationship between mechanical vibration and roots development of planflet. Methods： By mechanical vibrating Actinidia chinensis exophytes that came from their callus to imitate the environmental stress, and several important plant physiological indices involved in root development were investigated. Results： The proper mechanical vibration （i.e., 2-3 Hz） imposed on plantlet could promote their roots development and growth, the root activity, total length and amount of roots increased, whereas the permeability of cell membrane decreased. As the intensity （or frequency） of vibration exceeded certain limit value （4 Hz）, the increase of root activity would be restrained chiefly owing to the integrality of cell membrane being destroyed. Conclusion： The experiment showed that mechanical vibration has some dual effects on root development of woody plant.

关 键 词：机械振荡 中华猕猴桃 试管苗 根系活力 

分 类 号：Q943.1[生物学—植物学] Q689