严重急性呼吸综合征患者血清特异性抗体消涨规律的长期追踪研究  

Profile of Antibodies of SARS Coronavirus in 3 Years After Infection

作　　者：石玉玲 李亚南 李林海 段朝晖[2] 曹诚[3] 

机构地区：[1]广州军区广州总院检验科,广东广州510010 [2]中山大学附属第二医院,广东广州510000 [3]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2007年第1期91-92,共2页Letters in Biotechnology

摘　　要：目的:追踪检测SARS冠状病毒(SARS-CoV)抗体在严重急性呼吸综合征(SARS)患者血清中的产生及其转归规律,为SARS诊断及防治提供依据。方法:对41例临床诊断SARS患者的血清进行了连续3年的检测,分别应用间接免疫荧光(IFA)检测患者血清特异性IgG抗体平均滴度,应用双抗原夹心ELISA法检测患者血清核衣壳蛋白(N蛋白)抗体的平均滴度,绘制消涨曲线,得出消涨规律。结果:应用IFA检测患者血清特异性IgG抗体与应用双抗原夹心ELISA法检测N蛋白抗体所得到的消涨规律不同,前者测得康复者血清IgG抗体滴度维持在较低水平,但后者检测35例康复者血清N蛋白抗体仍维持在较高水平。结论:SARS-CoV的N蛋白是免疫原性较强的抗原,感染3年后仍存在高滴度抗体;抗原夹心ELISA检测SARS-CoV N蛋白抗体的灵敏度较IFA方法高。Objective： Access the antibody response profile after severe acute respiratory syndrome（SARS） coronavirus （SARS-CoV） infection. Methods： Antibodies against SARS-CoV or the nucleocaspid protein（N protein） of the virus in 41 SARS patients were continually monitored for 3 years by indirect fluorescence assay or antigen-capturing ELISA. Results： Antibody against SARS-CoV and it N protein could be detected in 35 out of 41 cured SARS infected people by the two method, while the sensitivity was much higher in later phase by antigen-capturing ELISA. Conclusion： The N protein is the major antigen of SARS-CoV. Antibody in human can last more than 3 years. The sensitivity of antigen capture ELISA is higher than IFA.

关 键 词：严重急性呼吸道综合征 核衣壳蛋白 免疫荧光 抗体 

分 类 号：R392.1[医药卫生—免疫学]