标准化嵌合体制备体系的研究  

Study on Standardization System for Chimeras Production

作　　者：何志颖[1] 王新民[1] 李建秀[1] 胡以平[1] 

机构地区：[1]第二军医大学细胞生物学教研室,上海200433

出　　处：《生物技术通讯》2007年第2期213-216,共4页Letters in Biotechnology

基　　金：国家高技术研究发展计划项目(102-10-04-03);国家自然科学基金项目(30270603)

摘　　要：目的:完善和规范基因剔除小鼠技术体系的关键技术环节,建立一套高效的嵌合体制备体系。方法:优化胚胎干细胞(ES细胞)的基本培养条件;应用条件培养液筛选、富集高嵌合潜能的ES细胞;成熟嵌合体的制备技术;改变胚胎的移植方式,改善受体的生理状态。结果:ES细胞基本培养条件的优化及条件培养液的筛选保持了ES细胞的整体高嵌合潜能,嵌合体制备技术得以成熟,胚胎移植方式的改变提高了移植胚胎的产仔率,这些措施大大提高了嵌合体的制备效率。结论:通过对基因剔除小鼠技术体系的关键技术环节进行优化和改进,建立了一套高效的嵌合体制备程序,为基因剔除小鼠服务体系的开展打下了坚实的基础。Objective： To establish a highly efficient production system of chimeras by perfecting and normalizing the key steps of technology system of gene knockout mice. Methods： To optimize the basic cultivation conditions of ES cells; to screen and enrich the high chimeric potential of ES cells by using conditional cultivation medium; to improve the production efficiency of chimeras by maturing correlated technique; to improve the state of recipient mice by changing the method of embryo transplantation. Results＇ High chimeric potency of ES cells was maintained by optimization of basic cultivation conditions and use of conditional cultivation medium. The production technology of chimeras was mature, and the fetiferous rate was improved by changing the method of embryo transplantation. These measures increased the production efficiency of chimera remarkably. Conclusion： This study established a highly efficient production system of chimeras by perfecting and normalizing the key steps of technology system of gene knockout mice, which has laid a solid foundation for offering the serve of gene knockout mice technology.

关 键 词：嵌和体 胚胎干细胞 条件培养液 基因剔除小鼠 

分 类 号：Q78[生物学—分子生物学]