肾脏缺血后处理对家兔缺血再灌注心肌酶的影响  

EFFECTS OF RENAL ISCHEMIC POSTCONDITIONING ON THE MYOCARDIUM ENZYMES IN RABBITS WITH ACUTE MYOCARDIAL ISCHEMIA AND REPERFUSION

作　　者：张文忠[1] 陈文一[1] 刘松[2] 蔡尚郎[2] 

机构地区：[1]青岛市妇女儿童医疗保健中心,山东青岛266011 [2]青岛大学医学院附属医院心内科

出　　处：《齐鲁医学杂志》2007年第3期204-205,208,共3页Medical Journal of Qilu

摘　　要：目的研究肾脏缺血后处理对家兔急性缺血再灌注心肌酶的影响,并探讨其机制。方法24只新西兰大白兔随机分为3组,每组8只。对照组结扎冠状动脉前降支1 h,再灌注6 h。实验组于再灌注时对左侧肾动脉行结扎30 s、开通30 s的3次反复循环,余同对照组。药物组于再灌注前10 min静脉注射蛋白激酶C抑制剂GF109203X,余同实验组。分别于结扎前、再灌注3 h、再灌注6 h采取兔血,测定各组血肌酸激酶同工酶(CK-MB)和肌钙蛋白(cTNI)的水平。实验结束时NBT染色测定心肌梗死面积。结果与对照组相比,实验组在再灌注各时间点CK-MB活性和cTNI浓度均降低,实验组坏死面积占总面积之比小于对照组;GF109203X完全取消了上述保护作用。结论肾脏缺血后处理对缺血心肌具有保护作用,其机制与蛋白激酶C激活有关。Objective To investigate the effects of renal ischemic postconditioning （Renal PostC） on myocardium enzymes in rabbits with acute myocardial ischemia and reperfusion. Methods Twenty four Newzealand rabbits were divided into three groups. Control group： the rabbits underwent 60 minutes of left coronary artery occlusion （LCAO） and six hours of reperfusion; Renal PostC group： after 60 minutes of LCAO, the left renal artery of the rabbit repeated three times of 30-second occlusion and 30-second release during coronary artery reperfusion period; Drug group： after 60 minutes of LCAO, the rabbits received 0.05 mg/kg Ⅳ of PKC antagonist GF109203X, again the left renal artery repeated three times of 30-second occlusion and 30-second release during coronary artery reperfusion. Blood samples were taken before LCAO, 3 h and 6 h after reperfusion for CK-MB and cTNI determination. The myocardium infarction size （IS） was measured with NBT dye at the end of the experiment. Results Compared with the control group, The levels of CK-MB and cTNI in PostC group was significantly reduced at 3h and 6h after reperfusion （P〈0.05）, the IS percentage （infarction area/total areas） was also significantly （P〈0.05 ） reduced in Renal PostC group. F109203X abolished the protective effect of Renal PostC. Conclusion Remote renal postconditioning applied immediately before the onset of coronary artery reperfusion protects ischemia myocardium. This inter-organ remote postconditioning phenomenon is likely mediated in part by activation of PKC by the ischemic-renerfused kidney.

关 键 词：肾 缺血后处理 再灌注损伤 心肌酶 兔 

分 类 号：R542.22[医药卫生—心血管疾病] R364.12[医药卫生—内科学]