养殖乌鳢类立克次体感染的组织病理学研究  

HISTOPATHOLOGICAL STUDIES ON RICKETTSIA-LIKE ORGANISM INFECTION IN A CULTURED FISH,OPHIOCEPHALUS ARGUS

作　　者：郭琼林[1] 贾伟章[1] 孙晓凤[1] 周秀霞[1] 李海燕[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072

出　　处：《水生生物学报》2007年第1期45-52,共8页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(No.30371091)资助

摘　　要：本文系统报道了养殖乌鳢类立克次体(Rickettsia-like organism,RLO)感染的各器官(脑、眼、鳃、心脏、头肾、肝、胰腺组织、脾、肾、肠和卵巢)的组织病理变化,探讨了炎症发展的基本规律。感染乌鳢病理解剖学特征和最具病理诊断意义的是体内各器官普遍出现的白色结节。这些结节的显微结构为肉芽肿炎症即一种慢性增生性炎症。在严重病变的肾脏,由于组织坏死区域较大和周围明显的细胞增生形成了境界较为清楚的巨大“肉瘤”状肿物。内脏器官的血管(特别是造血器官的血管)出现明显纤维素性血栓、混合血栓、弥散性血管内凝血(disseminated intravascu-lar coagulation,DIC)和组织细胞大范围的变性或坏死、溶解。大多数器官的组织细胞主要是上皮性细胞、吞噬性细胞,胞质内富含嗜酸性包涵体(eosinophilic intracytoplasmic inclusion),这种细胞多位于鳃上皮处、鳃部和体内器官血管内皮及血管周边结缔组织。血管内皮细胞及周边细胞内大量包涵体的出现导致血管内皮细胞的肿胀和破坏。The histopathological changes of various organs （ brain, eye, gill, heart, head kidney, liver, hepato-pancreas, spleen, kidney, intestine and ovary） in rickettsia-like organisms （RLOs） infected fish, Ophiocepha1us argus C. have been reported,and the pathological mechanism has been discussed in this paper. The gross pathological characteristics were that cream-coloured nodules, a chronic granulomatous inflammation under microscopy, were observed on internal organs of infected fish, which had diagnostic significance. Because of extensive tissue necrosis and marked fibroblast proliferation,5—10 giant sarcoma-like bodies with a distinctive boundary were seen on the surface of severe infected kidney. The blood vessels of internal organs showed fibrin thrombi, mixed thmmbi and disseminated intravascular coagulation （DIC）, and larger areas of tissue and cells showed severe degeneration, necrosis and extensive lysis. Eosinophilic intracytoplasmic inclusion-laden cells,which were mainly epithelial cells and phagocytic cells,situated mostly in the epithelium of gill, the endothelium of blood vessels and the connective tissue near to the blood vessels of gill and internal organs. The intracytoplasmic inclusions could result in swelling and rupturing of endothelial cells of blood vessels. The histopathological changes of various organs observed in experimentally infected fish with abundant RIDs were similar to those in naturally infected fish. The marked necrosis and larger granulomas were found in brain tissue,fibrin thrombi were also observed in blood vessels of brain membrane. The central necrotic area of granulomas in eye was larger. The gill lamellae were swollen and ruptured, epithelial cells showed necrosis and sloughed. The myocarditis and pericarditis were seen in heart. The severe changes were noted in the kidney. The kidney changes were characterized by the replacement of normal haematopoietic tissue with a large number of granulomas in different size. The renal tubules showed widespread degeneratio

关 键 词：类立克次体 鱼病 鱼类病理学 乌鳢 

分 类 号：S941.1[农业科学—水产养殖]