声电子胰岛素免疫传感器固定化活性抗体膜的制备研究  

Preparation of Immobilized Antibody Membrane of Acoustic Immunosensor for Determination of Insulin

作　　者：陈建华[1,2,3] 顾文照[1,2,3] 郭雅彬 丁黎章德 李有志 

机构地区：[1]中国药科大学生物化学教研室 [2]医药电子仪表教研室 [3]南京大学近代声学实验室

出　　处：《中国药科大学学报》1997年第1期52-52,共1页Journal of China Pharmaceutical University

基　　金：江苏省科学技术委员会资助

摘　　要：选择５％聚乙烯醇为膜材料，与１０ｕ／ｍｌ胰岛素抗体混匀，在压电石英晶体上制备活性抗体膜。放免法检测结果表明，抗体膜具有良好的机械强度、透性和响应性能。抗体膜与不同浓度的胰岛素抗原结合，显示抗体膜上胰岛素结合量与胰岛素浓度呈线性相关。Active antibody membrane on piezoelectric quartz crystal was prepared with the mixture of 5% polyvinyl alcohol(PVA) and 10 u/ml insulin antibody. The results measured by radioimmunoassay and acoustic immunosensor show that there are better functions in the strength, responsibility, and penetration of the membranes . The binding amount of insulin to the certain concentration of antibody immobilized in the membrane is better correlative to the concentration of insulin in samples.

关 键 词：免疫传感器 胰岛素 抗体膜 聚乙烯醇 

分 类 号：R393－33[医药卫生—基础医学] R977.15