结核病房空气中结核分支杆菌检测方法研究  

Methods for detecting Mycobacterium tuberculosis in the air of wards for tuberculosis patients

作　　者：张本[1] 王忠仁[1] 童小玫 吴兆儒[1] 宛淑贤 

机构地区：[1]北京市结核病胸部肿瘤研究所

出　　处：《中华结核和呼吸杂志》1997年第2期101-103,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的探索空气中结核分支杆菌检测的有效方法。方法选用ＬＷＣ－Ⅰ型空气微生物采样器，对菌阳肺结核病房进行空气采样，３０份采样标本分别用不同方法检测结核分支杆菌。结果聚合酶链反应（ＰＣＲ）阳性１４份，阳性率４７％；Ｓｏｕｔｈｅｒｎ转印杂交阳性１８份，阳性率６０％；动物接种实验组３０只豚鼠有２只结核分支杆菌培养阳性，另１只病理组织学检查阳性，而对照组１０只豚鼠脏器培养及病理组织学检查全部阴性；罗氏培养和ＢＡＣＴＥＣ快速培养均呈阴性。结论用空气采样的方法进行空气中结核分支杆菌的监测是可行的。ＰＣＲ和Ｓｏｕｔｈｅｒｎ转印杂交最敏感，动物实验也可提供有意义的参考。ObjectiveTo explore useful methods for detecting M.tuberculosis in the air of inpatient′swards of tuberculosis.Methods Thirty samples were collected by using LWC Iair sampler from the air of wards housing sputum positive patients.The samples were washed with0.85%sodium chloride solution,then centrifuged,and finally detected M.tuberculosis by L Jmedium culture, BACTEC method,guinea pig experiment,PCR and Southern blotting.Results The positive rates by PCR and Southern blotting were47%(14/30)and 60%(18/30)respectively.In the experimental group containing 30 guinea pigs,two were positive by culture from the animal tissues(spleens or lungs),and another one was pathologically positive.Ten guinea pigs in

关 键 词：结核分支杆菌 空气微生物学 结核病房 

分 类 号：R117[医药卫生—公共卫生与预防医学]