牙周细胞在离子化胶原表面的黏附与生长  

Periodontal cell ingrowth and adhesion on the surface of ionized collagen

作　　者：陈泽红[1] 张焰[1] 王永宏[2] 蒋波[1] 顾忠伟[1] 

机构地区：[1]四川大学生物材料工程研究中心,四川省成都市610064 [2]四川大学生命科学院,四川省成都市610064

出　　处：《中国组织工程研究与临床康复》2007年第26期5133-5136,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:评价离子化胶原对不同交联剂的作用及交联后对牙周细胞的黏附与生长的影响。方法:实验于2006-10/2007-01在四川大学生物材料工程研究中心实验室完成。①通过胶原蛋白与甲醇在低温反应得到化学修饰离子化胶原,将胶原海绵和离子化胶原海绵分别与戊二醛、碳化二亚胺,京尼平在室温条件下发生交联反应,控制交联剂的终质量分数为0.006,交联时间均为24h。②通过测溶液中羟脯氨酸含量测交联产物的交联度;用胶原酶测交联产物体外降解时间;通过牙周细胞在交联产物上的生长情况(A570)确定交联产物对细胞的作用。nm结果:①吸液率和交联度:离子化胶原与各交联剂交联后吸液率都低于胶原交联后,交联度相应提高,胶原和离子化胶原与戊二醛交联其交联度最高,分别为81%,85%。②降解率:离子化胶原的降解率总的趋势低于胶原样品,离子化胶原、胶原被炭化二亚胺交联后,其降解率分别为0.5%,45.0%。③牙周细胞生长情况:离子化胶原交联产物上牙周细胞A570值高于胶原交联nm产物。结论:离子化胶原在交联过程中显示了较好的抗生物降解性能,离子化胶原交联产物在牙周细胞培养过程中显示出了低的细胞毒性,提示其对牙周细胞黏附与生长具有促进作用,可以代替胶原应用于牙周组织治疗。AIM： To evaluate the effects of ionized collagen crosslinked with different cross-linking agents and analyze periodontal cell ingrowth and adhesion on the ionized collagen after cross-linking. METHODS： The experiment was conducted in the Engineering Research Center in Biomaterials, Sichuan University from October 2006 to January 2007.①Native collagen was modified at low temperature by chemical methods using methanol to obtain the ionized collagen, then collagen sponge and ionized collagen sponge were crosslinked with glutaraldehyde （GTA）, 1-ethyl-3-3-dimethylaminopropyl carbodimmide hydrochloride （EDC） and genipin at the environment temperature, the concentration of the cross-linking agent was controlled to 0.006, and the time of crosslink reaction was 24 hours.②Ninhyddn was used to evaluate the cross-linking index. The degradation rate of crosslinked samples in vitro was determined by using collagen enzyme, the viability of periodontal cells （A570） on the crosslinked samples were measured to analyze the effects of the samples to the cells. RESULTS： ①The swelling ratio of the ionized collagen was decreased compared with that of collagen after cross-linking, while the cross-linking index was increased and reached a peak when crosslinked with GTA （81%, 85%）. ②The degradation rate of ionized collagen was lower than that of collagen samples. The degradation rate of ionized collagen crosslinked with EDC was only 0.5% while that of collagen crosslinked with EDC was 45.0%,③Ingrowth of peritoneal cells： The A570nm value of periodontal cells on ionized collagen was higher than that on the samples of collagen. CONCLUSION： Ionized collagen after cross-linking has better anti-biodegradation and lower cytotoxicity, indicating that ionized collagen accelerates the ingrowth and adhesion of periodontal cells, and it can be applied in the periodontal therapy instead of collagen.

关 键 词：胶原 离子化胶原 交联反应 交联度 牙周细胞 

分 类 号：R318.08[医药卫生—生物医学工程]