丝素蛋白在组织工程中的应用  

Silk fibroin in tissue engineering

作　　者：段巧艳[1] 段祥[1] 张焕相[1] 

机构地区：[1]苏州大学生命科学学院,江苏省干细胞研究重点实验室,江苏省苏州市215123

出　　处：《中国组织工程研究与临床康复》2007年第26期5199-5203,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家重点基础研究发展计划(九七三计划)(2005CB623906);江苏省高校自然科学研究计划(05KJB310115);江苏省丝绸工程重点实验室;苏州大学医学发展基金资助(EE120602)~~

摘　　要：目的:总结丝素蛋白在生物材料和组织工程领域的应用,分析和展望丝素蛋白材料在医学生物领域的应用前景。资料来源:应用计算机检索PUBMED 1993-01/2007-01关于丝素蛋白和组织工程的文章。检索词为“silk,tissue engineering”,并限定文章的语言种类为English。同时利用计算机检索中国期刊全文数据库1994-01/2007-01的相关文章,限定文章语言种类为中文,检索词为“丝素蛋白、组织工程”。资料选择:对资料进行初审,纳入标准:①丝素蛋白的性质、在组织工程中的应用。②骨髓间充质干细胞与丝素蛋白复合的相关性研究。③丝素蛋白的改性、修饰在生物学领域的应用。排除标准:重复性研究。资料提炼:共收集到符合上述要求的文献96篇,排除45篇重复性研究。48篇符合纳入标准:其中13篇关于丝素蛋白的结构和性质的,7篇关于丝素蛋白表面修饰的,篇关于丝素蛋白和组织工程的。28资料综合:实验证明丝素蛋白可以多种形式(薄膜、膜、网状结构、海绵状等)在体外支持干细胞的黏附、增殖和分化,体内促进组织的修复,尤其是干细胞组织工程中三维的丝素蛋白支架扩大了丝素作为生物材料的应用,成为最有前景的生物材料之一。结论:丝素蛋白作为一种新的生物材料应用越来越受到关注。而且,通过多种方法改进、表面修饰等技术控制丝素蛋白的分子结构和形态,使得丝素蛋白在生物材料和组织工程领域的应用变得更为广泛。OBJECTIVE： To summarize the application of silk fibroin in the biomaterials and tissue engineering, analyze and forecast the prospect of silk fibroin-based materials in the field of biomedicine. DATA SOURCES： A computer-based search of Pubmed was undertaken to identify the articles relevant to silk fibroin and tissue engineering from January 1993 to January 2007, with the key words ＂silk, tissue engineering＂ in English; Similarly, the relevant articles between January 1994 and January 2007 were searched in China Journal Full-text Database with the key words ＂silk fibroin, tissue engineering＂ in Chinese. STUDY SELECTION： The literatures were selected primarily. The criteria for selection were those about silk fibroin＇s property and its application in tissue engineering, those correlative studies about bone marrow stromal cells combined with silk fibroin, and those about the modification of silk fibroin for medical applications. Exclusion criteria： repetitive study. DATA EXTRACTION： There were 96 literatures in accordance with the criteria, 48 were selected and 45 of them were excluded due to the repetition of the same research. Among the selected literatures, 13 reviewed the structure and properties of silk fibroin, 7 studied the surface modification, and 28 researched silk fibroin＇s application in tissue engineering. DATA SYNTHESIS： Silk fibroin in various formats （films, nets, membranes, sponges and so on） has been shown to support stem call adhesion, proliferation and differentiation in vitro and promote tissue repair in vivo. In particular, stem call-based tissue engineering using three-dimensional silk fibroin scaffolds has expanded the use of silk-based biomaterials as one of the most promising scaffolds, CONCLUSION： Silk fibroin has been increasingly studied for its new biomedical application. Modification of silk fibroin controls its molecular structure and morphology and leads the silk flbroin-based materials for medical applications.

关 键 词：丝素蛋白 生物材料 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]