壳聚糖-明胶海绵状伤口敷料的制备及其性能特征(英文)  

Preparation and performance of chitosan-gelatin sponge-like wound-healing dressing

作　　者：何兰珍[1] 刘毅[1] 杨丹[1] 

机构地区：[1]广东海洋大学理学院,广东省湛江市524088

出　　处：《中国组织工程研究与临床康复》2007年第26期5252-5256,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省教育厅自然科学研究项目(0151);广东省自然科学基金资助项目(033984)~~

摘　　要：背景:壳聚糖具有良好的成膜性和黏度,壳聚糖含有自由氨基,可与明胶配位而交联,其分子间可以通过氢键形成天然的半互穿聚合物网络结构。目的:通过冷冻壳聚糖-明胶共混液诱导相分离的方法,制成多孔性、亲水性、透气性良好的海绵状伤口敷料。设计:观察对比分析。单位:广东海洋大学理学院实验室。材料:原料:壳聚糖(由湛江产海水养殖虾虾壳自制)脱乙酰度97.55%,=1.85×106;明胶,CP级,上海化学试剂分装厂;冰乙酸;NaOH;甲醛;甘油,均为CP级。设备:XL30-EDAX扫描电子显微镜(荷兰Philips生产);3365型万能材料试验机。方法:①通过冷冻壳聚糖-明胶共混液诱导相分离的方法,将壳聚糖质量分数为0.2,0.4,0.5,0.6,0.8溶液混合明胶溶液,经交联后制成多孔性、亲水性、透气性良好的海绵状伤口敷料。再加入少量甘油,混合均匀,静置脱泡,将样品按常规方法镀金,在扫描电镜上观察敷料表面结构与截面结构。观察不同壳聚糖与明胶比例对海绵体敷料各项性能参数(包括敷料保湿性、吸湿性、抗张强度及透气率)的影响。②以质量浓度18,20,22,25,24,26,28g/L壳聚糖-明胶制备敷料,观察不同含量条件对壳聚糖-明胶海绵体性能的影响。③以体积分数为0.002,0.004,0.006,0.008,0.010,0.012,0.014的甲醛为交联剂,观察不同交联剂用量对敷料撕裂强度影响。主要观察指标:①敷料表面结构与截面结构的观察结果。②不同壳聚糖与明胶比例对海绵体敷料各项性能参数的影响。③不同含量条件对壳聚糖-明胶海绵体性能的影响。④不同交联剂用量对敷料撕裂强度影响。结果:①用此方法制备的壳聚糖-明胶海绵状敷料,从表面可以清楚地看到材料为多孔结构;从截面看,孔是蜂窝结构或多孔片层堆积而成的三维立体片层结构。②当壳聚糖含量较大时,其截面接近蜂窝状,随着壳聚糖含量减少,明胶含量增大,截面�BACKGROUND：Chitosan has good filming and viscosity, it contains free amido, and can coordinate and cross-link with gelatin, thus natural semi-interpentrating polymer network structure can be formed among molecules through hydrogen bonds.OBJECTIVE： To prepare sponge-like wound-healing dressing of good porosity, hydrophilia and air permeability by means of frozen chitosan-gelatin mixture induced phase separation.DESIGN： A comparative observation.SETTING： Laboratory of the College of Science, Guangdong Ocean University. MATERIALS： Chitosan was deacetylated by 97.55%, Mη=1.85×106; Gelatin （CP grade） was produced by Shanghai Chemical Dispensing Factory; Glacial acetic acid, NaOH, formaldehyde and glycerin were all CP grade. XL30-EDAX scanning electron microscope （Philips, Dutch）; 3365-type universal material testing machine. METHODS： ① By means of frozen chitosan-gelatin mixture induced phase separation chitosan solution of 0.2, 0.4, 0.5, 0.6 and 0.8 （mass fraction） was mixed with gelatin solution, then a small amount of glycerin to prepare sponge-like wound-healing dressing of good porosity, hydrophilia and air permeability. then a small amount of glycerin, and stayed quietly to deaerate. The samples were plated with gold as routine methods, and then the surface and sectional structures were observed under the scanning electron microscope. The effects of different proportion of chitosan and gelatin on the performance parameters （water retention, moisture absorption, avulsion intensity, air permeability rate） of the sponge-like materials were observed. ② Chitosan-gelatin mixtures of 18, 20, 22, 25, 24, 26 and 28 g/L （mass fraction） were used to prepare sponge-like materials, and the effects of different contents on the performances of the materials were observed. ③ The effects of cross-linking agent （formaldehyde） of different dosages （0.002, 0.004, 0.006, 0.008, 0.010, 0.012 and 0.014 in volume fraction） on the avulsion intensity of the materials were observed.MAIN OUTC

关 键 词：壳聚糖 明胶 相分离 冷冻诱导 海绵状伤口敷料 

分 类 号：R318.08[医药卫生—生物医学工程]