三氯化镧对CBRH-7919细胞细胞周期素D_1和周期素依赖性激酶4蛋白表达的影响(英文)  

Effect of lanthanum chloride on the proliferation and expressions of cyclin D_1 and cyclin dependent kinase 4 in rat hepatocellular carcinoma CBRH-7919 cells

作　　者：杨立明[1] 姜文华[2] 郝利铭[2] 孟晓婷[2] 

机构地区：[1]吉林大学第一临床医院肾病科,吉林省长春市130021 [2]吉林大学基础医学院组织胚胎教研室,吉林省长春市130021

出　　处：《中国组织工程研究与临床康复》2007年第26期5257-5260,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：吉林省科技厅自然科学基金资助项目(20030542-1)~~

摘　　要：背景:国内外多年的研究表明稀土化合物在体内、外均具有明显的抑瘤性能。目的:观察三氯化镧对大鼠肝癌细胞细胞周期素D1和周期素依赖性激酶4蛋白表达的影响。设计:对照观察。单位:吉林大学第一临床医院肾病科。材料:实验于2002-06/2003-07吉林大学基础医学院组织胚胎教研室细胞培养室完成。CBRH-7919细胞按三氯化镧剂量不同分为4组,即空白对照组,0.01,0.1,1.0mmol/L三氯化镧组,每组分别培养1,3,5d,每组细胞各接种6复孔。方法:以体外培养大鼠肝癌细胞株CBRH-7919为研究对象,给予0.01,0.1,1.0mmol/L三氯化镧,培养后1,3,5d观察CBRH-7919细胞生长变化。①运用流式细胞术计算各期细胞所占比例。②四甲基偶氮唑蓝实验测定各孔吸光度值(A值)。③免疫细胞化学检测与G1期调控有关的细胞周期素D1和周期素依赖性激酶4的变化情况。主要观察指标:CBRH-7919细胞在不同剂量和不同培养时间的细胞周期素D1和周期素依赖性激酶4的表达情况。结果:①三氯化镧对CBRH-7919细胞生长的作用:0.1,1.0mmol/L三氯化镧组培养后3,5d吸光度值低于空白对照组(3d分别为1.140±0.070,0.706±0.092,1.461±0.087;5d分别为1.888±0.020,0.625±0.037,2.544±0.032),差异有显著性意义(P<0.05,0.001)。②三氯化镧对CBRH-7919细胞G0/G1期百分率的影响:1.0mmol/L三氯化镧组培养后1,3,5dG0/G1期细胞百分率高于空白对照组[分别为(60.70±0.20)%,(39.49±0.67)%;(61.66±0.97)%,(45.56±1.00)%;(69.92±0.18)%,(49.24±0.27)%],差异有显著性意义(P<0.01,0.001);0.1mmol/L三氯化镧组培养后5dG0/G1期细胞百分率高于空白对照组[分别为(58.88±0.73)%,(49.24±0.27)%],差异有显著性意义(P<0.05)。③三氯化镧对CBRH-7919细胞细胞周期素D1和细胞周期素依赖性激酶4阳性表达的影响:0.1,1.0mmol/L三氯化镧组培养后1d细胞周期素D1和细胞周期素依赖性激酶4表达(A)明显低于空白对照组(分别为562±35,453±22,860BACKGROUND： It has been proved that the rare earth compounds can obviously restrain the growth of tumor in vitro and in vivo.OBJECTIVE： To check the effect of chlorinated lanthanum （LaCl3） on the expressions of cyclin D1 and cyclin dependent kinase 4 （CDK4） of hepatocellular carcinoma cells.DESIGN： Controlled observation.SETTING： Department of Nephropathy, First Clinical Hospital, Jilin University. MATERIALS： The experiment was carried out in the cell culture laboratory （province-level） of Department of Histology and Embryology, Jilin University from July 2002 to June 2003. Wells of the microwell plate seeded with CBRH-7919 cells were randomly divided into 4 groups according to the dose of LaCl3： control, 0.01, 0.1, 1.0 mmol/L LaCl3. And each group was further divided into three subgroups according to the culture time： 1, 3, 5 days, with 6 wells for each.METHODS： The growth of CBRH-7919 cells was monitored following treated with 0.01, 0.1, 1.0 mmol/L LaCl3 for 1, 3, 5 days in vitro, respectively. ① Absorbance（A） of each well was measured by MTT assay.② The changes of distribution of cells into different phases of cell cycle were detected by flow cytometry. ③ Cyclin D1 and CDK4 expression was detected by immunocytochemical analysis.MAIN OUTCOME MEASURES： The expression of cyclin D1 and CDK4 in CBRH-7919 cells of different doses of LaCl3 at different time.RESULTS： Effect of LaCl3 on the growth of CBRH-7919： On days 3 and 5, the A value of each well in the 0.1 and 1.0 mmol/L LaCl3 groups was respectively significantly lower than that in the control group （day 3： 1.140±0：070, 0.706±0.092,1.461±0.087; day 5： 1.888±0.020,0.625±0.037,2.544±0.032, respectively） （P 〈 0.05,0.001 ）. ②Effect of LaCl3 on the percentage of CBRH-7919 cells at G0/G1 stage： On days 1, 3, and 5, the percentage of CBRH-7919 cells at G0/G1 stage in the 1.0 mol/L LaCl3 group was respectively significantly higher than that in the blank control group [（60.70±0.20）%, （39.49±0.

关 键 词：稀土元素 三氯化镧 肝癌细胞 细胞周期素D1 细胞周期素依赖性激酶4 细胞周期 

分 类 号：R318[医药卫生—生物医学工程]