细胞外基质蛋白1基因在病理性瘢痕中的差异表达  

Differential expression of extracellular matrix protein 1 gene in abnormal scars

作　　者：胡振富[1] 李晓平[2] 宋艳斌[3] 

机构地区：[1]南方医科大学南方医院急诊科,广东省广州市510515 [2]南方医科大学南方医院肝胆外科,广东省广州市510515 [3]南方医科大学分子生物研究所,广东省广州市510515

出　　处：《中国组织工程研究与临床康复》2007年第27期5266-5268,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:病理性瘢痕包括增生性瘢痕和瘢痕疙瘩,比较细胞外基质蛋白1基因在增生性瘢痕和瘢痕疙瘩中的表达差异并分析其意义。方法:实验于2003-05/2005-01在南方医科大学分子生物学研究所完成。病理性瘢痕标本来源于南方医院整形外科手术患者,正常皮肤组织来源于无瘢痕体质趋势及无免疫性疾病的包皮手术标本。术前均征求患者同意后方留取标本。抽提增生性瘢痕和瘢痕疙瘩与正常皮肤组织的总RNA,反转录并双色荧光标记,与含有细胞外基质蛋白1基因的人类基因表达谱芯片杂交,经荧光扫描、数据处理和统计学分析,比较细胞外基质蛋白1基因在3种组织中的表达差异。结果:①芯片杂交图像显示荧光信号强度较高,背景均一,符合重复性和可靠性的要求和分析标准。②细胞外基质蛋白1基因在瘢痕疙瘩组织中的表达为正常皮肤组织的3.32倍,而在增生性瘢痕中的表达仅为正常皮肤组织的1.09倍。结论:细胞外基质蛋白1基因在瘢痕疙瘩中高表达而在增生性瘢痕中无差异表达,提示细胞外基质蛋白1基因在瘢痕疙瘩中呈特异性表达。AIM： Abnormal scar is consisted of hypertrophic scar （HS） and keloid. This study is aimed to investigate the differential expression of extracellular matrix protein 1 （ECM1） gene in abnormal scar, and analyze their significance. METHODS： The experiment was carded out in the Institute of Molecular Biology, Southern Medical University from May 2003 to January 2005. Abnormal scar samples were obtained from the patients, who underwent plastic surgery in Nanfang Hospital. Normal skin tissue came from the prepuce surgical sample without scar diathesis and immunological disease. All the patients agreed to fetch samples before operation. The total DNA was extracted from HS, keloid and normal skins respectively, then reverse transcription and two-color fluoresce labeling were followed. The total RNA was hybridized with human cDNA microarrays including ECM1 gene, followed by fluorescent scanning, data processing and statistical analysis, in order to detect the differential expression of ECM1 in HS and keloid in comparison with normal skins. RESULTS： （1）The image of chip hybridization showed that florescence signal strength was high and background was uniform, which accorded with the criteria for repeatability and reliability. （2）Expression of ECM1 gene in keloid was detected 3.32 times, and 1.09 times in HS compared with that in normal skin tissue. CONCLUSION： ECM1 gene is over expressed in keloid whereas no expression is found in HS, indicating that ECM1 expression in keloid is at a specific manner.

关 键 词：瘢痕疙瘩 瘢痕 肥大性 细胞外基质蛋白质类 基因表达 

分 类 号：R619.6[医药卫生—外科学]