骨形态发生蛋白对去势大鼠股骨颈骨形态、骨密度和骨生物力学的影响  

Effect of bone morphogenetic protein on the bone morphology, bone density and biomechanical properties of femoral neck in ovariectomized rats

作　　者：汪玉海[1] 刘兴炎[1] 白孟海[1] 葛宝丰[1] 陈克明[1] 吕明波[1] 

机构地区：[1]解放军兰州军区兰州总医院创伤骨科,甘肃省兰州市730050

出　　处：《中国组织工程研究与临床康复》2007年第27期5273-5276,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金："十五"全军重大课题(01Z008)~~

摘　　要：目的:观察在去势大鼠股骨颈植入牛骨形成蛋白后其骨形态、骨密度及骨强度的变化。方法:实验于2006-06/12在解放军兰州军区兰州总医院骨科研究所完成。①实验材料:普通级3个月龄wistar雌性大鼠60只。骨形态发生蛋白冻干粉剂(从幼龄牦牛新鲜长骨皮质中提取)。②实验分组:以同一只大鼠左右肢体为对照,右侧为实验侧,左侧为对照侧。③大鼠摘除卵巢制作绝经后骨质疏松模型经双能X射线骨密度仪检测筛选出造模成功的44只大鼠,实验侧距大转子0.5cm处股骨颈植入牛骨形成蛋白冻干粉剂,对照侧植入牛血清白蛋白。④实验评估:术后4周、8周时麻醉下处死大鼠取材,应用苏木精-伊红染色,组织切片后光镜下观察、CT扫描、双能X射线骨密度仪及万能生物力学机观察大鼠股骨颈骨形态学、骨皮质厚度、骨密度及骨强度的变化。结果:纳入造模成功后的44只大鼠均进入结果分析。①股骨颈骨组织形态学观察:4周时实验侧股骨颈局部骨小梁完整、连续性尚好;对照侧股骨颈局部骨小梁稀少、连续性中断,不完整。8周时实验侧股骨颈骨小梁致密,数量增多、完整,连接成网状结构、分布均匀;对照侧股骨颈骨小梁稀疏、变细、间距变大,呈髓腔扩大。②骨皮质厚度、骨密度及生物力学测定:4周时实验侧和对照侧股骨颈皮质厚度无明显差异,骨密度及最大载荷有明显差异(P<0.05);8周实验侧较对照侧骨皮质厚度明显增加、骨密度、最大载荷均增高[皮质厚度:(2.632±0.042),(1.728±0.034)mm,骨密度:(0.210±0.026),(0.182±0.029)g/mm2,最大载荷:(97.2±8.1),(85.6±7.9)N,P均<0.05]。结论:植入牛骨形成蛋白可以提高去势大鼠股骨颈局部骨皮质厚度、骨密度及生物力学强度,这将可能为绝经后骨质疏松性股骨颈骨折的防治提供一种新的途径。AIM： To study the effect of bovine bone morphogenetic protein （bBMP） implantation on bone density and pathology and biomechanical properties of the femoral neck in ovariectomized rats. METHODS： The experiment was carried out in the Institute of Orthopedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA from June to December 2006. （1）Sixty three-month-old female Wistar rats were selected and ovariectomized to establish the models of postmenopausal osteoporosis. Finally 44 rats were chosen by Dual-energy X-ray absorptiometry and the right femoral neck was implanted with freezable bBMP powder （extracted from the fresh long cortical bone of young poephogus grunniens） 0.5 cm from greater trochanter as the experimental side, and the left femoral neck of the same rat was implanted with bovine serum albumin （BSA） as the control side. （2） Specimens were obtained at postoperative weeks of 4 and 8 after the rats were sacrificed under anesthesia. The morphology of femoral neck, thickness of cortex, bone density and biomechanical properties were given HE staining and tested by CT, Dual-energy X-ray absorptiometry and biomechanical test under light microscope. RESULTS： All the 44 rats entered the result analysis. （1）Bone morphology： At the 4^th week, the bone trabecula of expenmental femoral neck （the right side） was complete and good in consistency, while that of the left side was scarce, not consistent and incomplete. At the 8^th week, the bone trabecula of the experimental femoral neck grew densely and the quantity was increased and connected into net-shaped structure with even distribution. While the bone trabecula on the left side was scarce, and thinner with enlarged medullary cavity. （2）At the 4^th week, no difference was found in the thickness of cortex between the two sides, while the bone density and maximum load were significantly different （P 〈 0.05）. At the 8^th week, the cortex thickness, bone density and biomechanics of the right side were

关 键 词：骨形态发生蛋白质类 骨质疏松 绝经后 骨折 卵巢切除术 组织构建 

分 类 号：R618.4[医药卫生—外科学]