胰岛素样生长因子Ⅰ及其受体与梗阻肾纤维化的关系  

Relationship of insulin-like growth factor-Ⅰ and its receptor with obstructive renal fibrosis

作　　者：谢庆祥[1] 韩聪祥[1] 林吓聪[1] 

机构地区：[1]解放军第一七五医院泌尿外科厦门大学附属东南医院,福建省漳州市363000

出　　处：《中国组织工程研究与临床康复》2007年第27期5320-5323,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：福建省自然科学基金资助项目(Z0516082);漳州市科技计划基金资助项目(Z05068)~~

摘　　要：目的:检测胰岛素样生长因子Ⅰ及其受体在慢性梗阻性肾组织中的分布与表达,分析两者与梗阻肾纤维化之间关系,以进一步探讨两者在梗阻性肾病的发病过程中的作用。方法:实验于2005-06/11在解放军第一七五医院实验室完成。①实验材料:分别收集1991-01/2003-12因上尿路梗阻等原因行患肾切除标本47例和10例因其他原因行肾切除的正常肾组织,患者知情同意。②实验方法:对梗阻性肾组织和正常肾组织采用链菌素生物素-过氧化物酶标记物法进行免疫组织化学染色;采用Van Gieson苦味酸酸性复红染色法行胶原纤维染色。③实验评估:检测胰岛素样生长因子Ⅰ及其受体在梗阻性肾组织和正常肾组织中表达;并分析其表达与梗阻肾纤维化之间的关系。胰岛素样生长因子Ⅰ及其受体阳性细胞数分为4个等级。肾纤维化程度判断分为0～3级。结果:①胰岛素样生长因子Ⅰ及其受体在正常和梗阻肾组织中表达阳性率:胰岛素样生长因子Ⅰ在梗阻肾肾小管和间质中表达阳性率均高于在正常肾组织中表达[在肾小管中差异无显著性意义(P>0.05);在间质中P<0.01];胰岛素样生长因子Ⅰ受体在梗阻肾肾小管和间质中表达阳性率[在肾小管中差异无显著性意义(P>0.05);在间质中P<0.01]。②胰岛素样生长因子Ⅰ及其受体表达与梗阻性肾纤维化之间关系:梗阻肾的间质组织中胰岛素样生长因子Ⅰ表达随梗阻肾纤维化程度加重而增高,不同肾纤维化分级之间的间质中胰岛素样生长因子Ⅰ的表达差异有显著性意义(P<0.05),而不同肾纤维化分级之间的肾小管胰岛素样生长因子Ⅰ及其受体表达和间质胰岛素样生长因子Ⅰ受体表达相比较差异均无显著性意义(P>0.05)。结论:胰岛素样生长因子Ⅰ及其受体异常表达参与梗阻性肾病的病理生理过程,尤其在肾间质纤维增生过程中起着重要作用。AIM： To examine the distribution and expressions of insulin-like growth factor- Ⅰ （IGF- Ⅰ ） and its receptor （IGF- Ⅰ R） in chronic obstructive kidney, and to analyze their relationships with renal fibrosis, so as to explore the their roles in the progression of chronic obstructive nephropathy. METHODS： The experiment was conducted in the Laboratory of the 175 Hospital of Chinese PLA from June to November 2005. （1）Forty-seven paraffin-embedded specimens and 10 specimens with normal kidney tissue, which were excised due to upper urinary obstruction and other reasons, were collected from January 1991 to December 2003. The informed consent was obtained from the patients. （2）Immunohistochemistry methods were adopted to examine the expressions of IGF- Ⅰ and IGF- Ⅰ R in chronic obstructive kidney and normal kidney tissue. Van Gieson staining method was used for collagen and fibrin staining. Relationships of IGF- Ⅰ and IGF- Ⅰ R expressions with obstructive renal fibrosis were analyzed, respectively. The number of positive IGF- Ⅰ and IGF- Ⅰ R was classified into 4 grades, and renal fibrosis was into 0-3 grade. RESULTS： （1）Positive expression rates of IGF- Ⅰ in renal tubule and interstitium of obstructive kidney was higher than that in normal kidney （renal tubule： P 〉 0.05; interstitium： P 〈 0.01）; significant difference was not found in the positive expression rates of IGF- Ⅰ R in renal tubule between obstructive kidney and normal kidney tissue （P 〉 0.05）, but in interstitium （P 〈 0.01）. （2）Expressions of IGF- Ⅰ in interstitial tissue of obstructive kidney was elevated with the aggravation of renal fibrosis, and had significant differences between different fibrosis grades （P 〈 0.05）, while no significant difference was found in the expression of IGF- Ⅰ and IGF- Ⅰ R between renal tubular and interstitial tissue （P〉 0.05）. CONCLUSION： Abnormalities of IGF- Ⅰ and IGF- Ⅰ R expression are involved in the pathophysiological prog

关 键 词：肾疾病 胰岛素样生长因子Ⅰ 受体 IGF1型 组织构建 

分 类 号：R692[医药卫生—泌尿科学]