正常和骨质疏松模型动物不同部位及不同器官摄取^(18)F-NaF的差异对骨代谢评估的意义  

Evaluating the bone metabolism by analyzing the ^(18)F-NaF ingestion in different positions and organs between normal and osteoporosis animal models

作　　者：吴启跃 马雯[2] 余正红[3] 雷洋洋[4] 李钦宗[5] 

机构地区：[1]深圳市体育运动学校,广东省深圳市518131 [2]河南省人民医院小儿骨科,河南省郑州市450003 [3]南方医科大学临床解剖研究所,广东省广州市510515 [4]天津中医药大学第一附属医院肾内科,天津市300193 [5]南方医科大学中医药学院骨伤系,广东省广州市510515

出　　处：《中国组织工程研究与临床康复》2007年第27期5346-5349,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:利用18F-NaF进行药代动力学研究,观察正常动物和骨质疏松模型动物在不同部位、不同器官摄取18F-NaF的差异评估骨代谢的改变情况。方法:实验于2005-05/2006-02在南方医科大学中医药学院实验室和南方医科大学附属南方医院PET中心完成。①分组:选取清洁级3月龄SD大鼠20只,随机数字表法分为两组:对照组和激素组,每组10只。②干预:激素组用注射过量激素法制作大鼠骨质疏松模型,用0.2μL/g地塞米松磷酸钠注射液(广东南国药业有限公司)肌肉注射,2次/周(每周2,5注射);对照组用同等剂量的NaCl注射液(武汉健民药业集团十堰康迪制药有限公司)注射。两组均注射8周。③骨密度测定:干预8周后,应用美国NORLAND生产的XR-36型双能骨密度测量仪对两组大鼠进行扫描,部位包括股骨颈、脊柱和肱骨下段。④18F-NaF扫描及图像重建:干预8周后,两组大鼠尾静脉注射18F-NaF,利用GE公司产的正电子发射计算机体层摄影-CT进行全身扫描,观察各处骨骼及肌肉和血液对18F-NaF的摄取,计算骨骼与肌肉和血液的比值,并进行统计学分析。将扫描得到的数据经迭代重建后进行图像融合,得到冠状、矢状、横断面CT,PET及PET/CT融合图像,然后通过融合软件进行组间对比。结果:20只大鼠全部进入结果分析。①骨密度值:激素组大鼠股骨颈、脊柱(T4)处低于对照组[(0.083±0.007),(0.086±0.013)g/cm2;(0.129±0.014),(0.124±0.018)g/cm2;P均<0.05]。②骨骼与肌肉和血液摄取18F-NaF的比值:激素组股骨颈、胫腓骨、桡骨远端、肱骨下端等部位低于对照组(0.71±0.27,0.61±0.27,0.55±0.23,1.16±0.98;1.12±0.54,0.90±0.51,0.75±0.39,1.44±1.09,P<0.05)。结论:骨质疏松部位对18F-NaF的摄取值的改变反应了骨的代谢变化,提示18F-NaF作为显像剂的正电子发射计算机体层摄影技术可以无创伤的检测骨质疏松骨代谢的改变,为早期检测骨质疏松提供理论依据。AIM： With the research of ^18F-NaF on pharmacokinetics, this study was designed to analyze the difference in ^18F-NaF ingestion in different positions and organs between the normal and the osteoporosis animal models, and evaluate the alternant mechanism of bone metabolism. METHODS： The experiment was accomplished in the Laboratory of Faculty of Traditional Chinese Medicine, Southern Medical University and the PET Center of Nanfang Hospital from May 2005 to February 2006. （1）Twenty clean grade SD rats （3 months old） were selected and divided into control group and hormone group randomly, each contained 10 rats. （2） The osteoporosis animal model was made by excess intramuscular injecting glucocorticoid with 0.2 μL/g dexamethasone sodium phosphate injection, twice a week （every Tuesday and Friday）; The control group was given the equal volume of sodium chloride injection. Both groups were treated for 8 weeks. （3） Densitometry： After 8 weeks＇ intervention, XR-36 dual-energy bone densitometer, produced by Norland, was used to scan the two groups, including the neck of femur, vertebral column and inferior of humerus. （4）^18F-NaF scanning and image reconstruction： After 8 weeks＇ intervention, all the rats were tail intravenously injected with ^18F-NaF and totally scanned by positron computed tomography （PET） CT, produced by GE Corporation, to observe the ^18F-NaF ingestion in skeleton, muscle and blood, calculate the content ratio, and perform the statistical analysis. The obtained data were iterative reconstructed to get image fusion of CT, PET, PET/CT, which was visualized in the coronal, sagittal, and transverse levels. The data of two groups were also contrasted. RESULTS： Twenty SD rats were all involved in the result analysis.（1）The bone density of the neck of femur and vertebral column （T4） in hormone group was lower than those in control group [（0.083±0.007）, （0.086±0.013） g/cm^2; （0.129±0.014）, （0.124±0.018） g/cm^2, P 〈 0.05]. （2）The ^1

关 键 词：^18F-NaF 正电子发射计算机体层摄影-CT 骨质疏松症 骨代谢 

分 类 号：R681.4[医药卫生—骨科学]