肠缺血再灌注大鼠肺及血液白细胞介素6、白细胞介素8和肿瘤坏死因子α变化与辅酶Q_10的干预  

Interventional effect of coenzyme Q_10 on the lung and serum levels of interleukin 6, interleukin 8 and tumor necrosis factor alpha in rats with intestinal ischemia/reperfusion injury

作　　者：张树平[1] 胡涛[2] 栾海云[1] 李庆忠[1] 李淑翠[1] 刘金苹[1] 

机构地区：[1]滨州医学院药理学教研室,山东省烟台市264003 [2]滨州医学院免疫学教研室,山东省烟台市264003

出　　处：《中国组织工程研究与临床康复》2007年第27期5429-5431,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察大鼠肠缺血再灌注时肺及血液白细胞介素6、白细胞介素8和肿瘤坏死因子α的变化以及辅酶Q10的影响。方法:实验于2006-12/2007-02在滨州医学院药理学实验室和免疫学实验室(山东省重点学科三级实验室)完成。①实验分组:清洁级健康Wistar大鼠30只,体质量290～390g,随机数字表法分为假手术组、肠缺血再灌注组,辅酶Q10处理组(缺血再灌注+辅酶Q10),每组10只。②实验方法:建立肠缺血再灌注模型,钝性分离肠系膜上动脉的根部,假手术组不作其他处理;肠缺血再灌注组再灌前30min时经股静脉注入生理盐水10mL/kg;辅酶Q10处理组再灌前30min时经股静脉注入辅酶Q1010mg/kg。③实验评估:酶联免疫吸附法(ELISA)检测各组动物血液、肺组织匀浆及肺泡灌洗液中的白细胞介素6、白细胞介素8和肿瘤坏死因子α含量;光镜下观察肺组织形态学变化。肺泡灌洗液沉渣进行白细胞计数和分类。结果:纳入大鼠30只,均进入结果分析。①肺组织形态学变化:假手术组肺组织无病理改变;肠缺血再灌注组肺间质明显水肿,中性粒细胞浸润,有少量的出血和纤维蛋白渗出;辅酶Q10处理组肺间质轻度水肿,少量的中性粒细胞。②肺泡灌洗液白细胞计数组间无显著性差异,多形核细胞分类肠缺血再灌注组明显高于假手术组(P<0.05),而辅酶Q10处理组与其他两组间差异无显著性意义(P>0.05)。③各组动物血液、肺组织匀浆及肺泡灌洗液中的白细胞介素6、白细胞介素8和肿瘤坏死因子α含量:与假手术组比较,肠缺血再灌注组血液、肺组织匀浆及肺泡灌洗液中白细胞介素6、白细胞介素8和肿瘤坏死因子α显著升高(P<0.05或P<0.01)。与肠缺血再灌注组比较,辅酶Q10处理组血液、肺组织匀浆和肺泡灌洗液中白细胞介素6含量显著降低(P<0.05);白细胞介素8和肿瘤坏死因子α含量在血液中显著降低(P<0.05);而在肺组织匀浆中�AIM： To observe the levels of interleukin 6, interleukin 8 and tumor necrosis factor α in lung and blood during intestinal ischemia/reperfusion in rats and the effect of coenzyme Q10. METHODS： The experiment was performed in Pharmacology and Immunology Laboratory of Binzhou Medical College from December 2006 to February 2007. （1）Thirty Wistar rats with the body mass of 290-390 g and of clean grade were randomly divided into sham operation group, intestinal ischemia/reperfusion group and the coenzyme Q10 treatment group （ischemia/reperfusion plus coenzyme Q10） with 10 in each group. （2）Intestinal ischemia/reperfusion models were established, and root of superior mesenteric artery were treated with blunt dissection. Sham operation group had no treatment. The rats were injected with saline 10 mL/kg through femoral vein in intestinal ischemia/reperfusion group, and the rats were injected with coenzyme Q10 10 mg/kg in coenzyme Q10 treatment group 30 minutes before the reperfusion. （3）Levels of interleukin 6, interleukin 8 and tumor necrosis factor α in blood, lung tissue plasma and alveolar lavage liquid of each group were determined by enzyme-linked immunosorbent assay （ELISA）. Lung histomorphological changes were observed by light microscope. The total and differential white blood cells counting in bronchoalveolar lavage fluid （BALF） in all groups were evaluated. RESULTS： Totally 30 rats were involved in the result analysis. （1）Histomorphological change of lung： No pathological change occurred in sham operation group. Visible pulmonary interstitial edema, neutrophil infiltration, a little hemorrhage and fibrin effusion were seen in the intestinal ischemia/reperfusion group. Mild pulmonary edema and little neutrophil infiltration were seen in the coenzyme Q10 treatment group. （2）The counting of white blood cells in bronchoalveolar lavage fluid （BALF） was no significant difference in each group. The counting of polymorphocyte was markedly higher in the intestinal ischemi

关 键 词：辅酶Q10 缺血再灌注 白细胞介素6 白细胞介素8 肿瘤坏死因子Α 

分 类 号：R392.114[医药卫生—免疫学]