以钙磷骨水泥为载体重组人血管内皮细胞生长因子与重组人骨形态发生蛋白-2复合体对异体脱蛋白骨修复骨关节缺损的促进效应(英文)  

Calcium phosphate cement attaching with recombinant human vascular endothelial growth factor and recombinant human bone morphogenetic protein-2 promotes deproteinized osteoarticular allograft to repair osteoarticular defect

作　　者：瞿向阳[1] 蒋电明[1] 安洪[1] 

机构地区：[1]重庆医科大学附属第一医院骨科,重庆市400016

出　　处：《中国组织工程研究与临床康复》2007年第27期5436-5439,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:脱蛋白骨具有与宿主骨相似的生物组织,有利于骨细胞附着的三维支架,有助于新生毛细血管的长入和间质细胞的迁入,钙磷骨水泥是一种新型的可降解的、非陶瓷型骨水泥,其理化特性具有可塑性,反应不产热且具有一定的力学强度和孔隙率。目的:观察钙磷骨水泥复合重组人血管内皮细胞生长因子(rhVEGF)与重组人骨形态发生蛋白-2(rhBMP-2)异体对脱蛋白骨关节移植修复骨关节缺损的促进作用。设计:随机对照观察。单位:重庆医科大学附属第一医院骨科。材料:选用36只成年杂交犬,雌雄不限,体质量(10±0.5)kg,实验动物由重庆医科大学动物中心提供。rhVEGF由北京博奥森生物技术有限公司提供。rhBMP-2由广州市达晖生物技术有限公司提供。钙磷骨水泥由上海瑞邦生物科技有限公司提供。方法:实验于2006-03/09在重庆医科大学临床学院骨科实验室(市级)完成,取36只实验犬,随机数字表法将杂交犬分成3组,每组12只。A组:钙磷骨水泥/rhVEGF/rhBMP-2/脱蛋白骨关节移植组;B组:rhVEGF/rhBMP-2/脱蛋白骨关节移植组;C组:单纯脱蛋白骨关节移植组。麻醉实验犬,距关节面30mm处用线锯切断股骨,用交叉克氏针和钢丝将各组相应移植物固定于股骨断端,逐层缝合切口。以上4项检查均在术后4,8,12,16周进行。术后4,8,12,16周进行移植物X射线、组织形成学、股动脉新生血管分布及膝关节血流量检测,并对术后16周各组实验犬骨关节变曲应力进行分析。主要观察指标:①移植物X射线、组织形态学、股动脉新生血管分布及膝关节血流量检测结果。②各组实验犬骨关节弯曲应力分析结果。结果:纳入实验犬42只均进入结果分析。①术后4～6周,从X射线检查发现A组骨痂形成明显好于B、C组。②苏木精-伊红染色显示A组在各时间点再血管化均优于B、C两组。免疫组织化学图像分析显示术后4、8、12及16周BACKGROUND： Deproteinized bone has three diamensions frame profitting bone cells to adhere to, new capillaries and interstitial cells to enter, osteoblasts to differentiate and extracellular matrix to synthesize. Calcium phosphate cement （CPC） is a new in-ceramic bone cement that can degradate, which has plasticity, no heat production, invariably mechanics intension and porosity.OBJECTIVE： To explore the ability of CPC attaching with human vascular endothelial growth factor （rhVEGF） and recombinant human bone morphogenetic protein-2 （rhBMP-2） in promoting deproteinized osteoarticular allograft to repair osteoarticular defect.DESIGN： Randomly control observation.SETTING： Department of Orthopaedics, the First Affiliated Hospital, Chongqing Medical University.MATERIALS： A total of 42 adult hybridization dogs of both genders and weighing （10±0.5） kg were provided by Animal Center of Chongqing Medical University. RhVEGF was provided by Beijing Boaosen Biotechnology Co., Ltd.; rhBMP-2 by Guangzhou Dahui Biotechnology Co., Ltd.; CPC by Shanghai Ruibang Biotechnology Co., Ltd.METHODS： The experiment was carried out in the Laboratory of Orthopaedics （Municipal Laboratory）, Clinical College,Chongqing Medical University from March to September 2006. A total of 36 adult dogs were randomly divided into three groups with 12 in each group, including group A： CPC/rhVEGF/rhBMP-2/deproteinized femoral inferior extremity （DPB）osteoarticular allograft; group B： rhVEGF/rhBMP-2/DPB osteoarticular allograft; group C： simple DPB osteoarticular allograft. The experimental dogs were cut off femur by wire saw from articular surface with 30 mm, then fixed the grafts to broken ends of fractured femur with cross Kirschner wires, drawn down the proximad periosteum and sutured it. At the 4^th, 8^th, 12^th and 16^th weeks after operation, the effects were partly assessed by X-ray film examination, histologic examination as well as immunohistochemistry analysis of VEGF and BMP-2, vas capillare analysis b

关 键 词：钙磷骨水泥 脱蛋白骨 血管内皮细胞生长因子 骨形态发生蛋白 骨关节移植 

分 类 号：R684[医药卫生—骨科学]