高效液相色谱法测定荣骨颗粒中淫羊藿苷的含量  

Determination of Icariin in Ronggu Granule by HPLC

作　　者：张秀梅[1] 王金[1] 何薇[2] 

机构地区：[1]首都医科大学中医药学院,北京100013 [2]北京市中医研究所,北京100010

出　　处：《中国实验方剂学杂志》2007年第2期10-11,共2页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:应用高效液相色谱(HPLC)法测定荣骨颗粒中淫羊藿苷的含量,控制该制剂的质量。方法:采用Zorbax-SB-C18柱(5μm,4.6×150 mm)分离淫羊藿苷,以甲醇-水(60∶40)为流动相,检测波长为270 nm。结果:淫羊藿苷线性范围为0.1-1.0μg,r=0.999 9,平均回收率为101.7%,RSD=1.86%(n=5)。结论:该方法简便、可靠、准确,可用于该制剂的质量控制。Objective： To determine the content of Icariin in Ronggu Granule by HPLC for quality control. Methods ：Zorbax-SB-C18（5 μm,4.6 × 150 mm） column was used with methanol： water （60：40）as mobile phase, and the determination wavelength was set at 270 nm. Results： The linear range was 0.1 - 1.0 μg for Icariin（ r = 0.999 9）, the average recovery was 101.7% and RSD was 1.86% （n = 5）. Conclusion：This method is simple, reliable and accurate for the determination of icariin, it can be used for quality control of this preparation.

关 键 词：荣骨颗粒 淫羊藿苷 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]