甘草桔梗皂苷对酪氨酸酶抑制的合并效应研究  

Evaluating the Combinative Effects of the Inhibition on Tyrosinase by Two Chinese Traditional Herbs:Platycodon Grandiflorum A.DC and Glycyrrhiza Uralensis Fisch

作　　者：宋杨[1] 齐云[1] 刘彬[1] 蔡润兰[1] 谢忱[1] 罗秀珍[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《中国实验方剂学杂志》2007年第3期7-10,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市自然基金资助课题(7042039);国家中医管理局资助课题(04-05ZL04)

摘　　要：目的:考察不同比例桔梗和甘草皂苷合用对酪氨酸酶的抑制作用。方法:改进传统的分光光度法,在微孔板上测定甘草、桔梗皂苷及二者的不同配比对酪氨酸酶的抑制活性,并采用国外常用的等高线和改良的等高线法对甘草桔梗不同配比的合用作用进行评价。结果:随着配比中甘草皂苷含量的增多,药物对酪氨酸酶的抑制活性从拮抗、相加到协同。结论:配比直接影响两药合用的效果。Objective：To evaluate the inhibitive effects on tyrdhsinase by the combinations of Chinese traditional herbs： Platycodon grandiflorum A. DC and Glycyrrhiza uralensis Fisch, mainly focusing on the differences of the doseeffect curves when changing the combination ratio of the two herbs. Methods： With isobolographic plot and the method developed by Tallarida RJ and the 96-well assay method on tyrosinase, the inhibitive effects of tyrosinase by the saponins of Platycodon grandiflorum A. DC and Glycyrrhiza uralensis Fisch were investigated. In the same time, fixed combinations of Platycodon grandiforus saponins （PS） and Glycyrrhiza uralensis saponins （GS） were also studied to give our a detailed picture of the combinative effects of the two herbs. Results ： With increasing of GS, the combination ratio has a great influence on the inhibitive effects of tyrosinase by the two herbs.

关 键 词：桔梗皂苷 甘草皂苷 酪氨酸酶 合用 

分 类 号：R285.5[医药卫生—中药学]